Tutorials

Load the Package and Data

devtools::install_github("dionnecargy/pvsero") # To download the package
library(pvsero) # To load the package 
library(tidyverse) # for data wrangling and visualisation
library(knitr)    # for RMarkdown visualisation and PDF generation
library(targets) # for data visualisation of pipeline (below)

Data Analysis: runPvSeroPipeline()

Run this global function runPvSeroPipeline() embedded within the {pvsero} R package! This function contains all of the steps in order of how to perform the Plasmodium vivax serology test and treat protocol as found in our application!

Using Tutorial Dataset: Load the Data

We will be using the build-in files in the R package for this tutorial, as shown below.

your_raw_data <- c(
  system.file("extdata", "example_MAGPIX_plate1.csv", package = "pvsero"),
  system.file("extdata", "example_MAGPIX_plate2.csv", package = "pvsero"), 
  system.file("extdata", "example_MAGPIX_plate3.csv", package = "pvsero")
)
your_plate_layout <- system.file("extdata", "example_platelayout_1.xlsx", package = "pvsero")

To run your OWN data, follow the code below and uncomment (i.e., remove the hashtags):

# your_raw_data <- c(
#   "PATH/TO/YOUR/FILE/plate1.csv",
#   "PATH/TO/YOUR/FILE/plate2.csv",
#   "PATH/TO/YOUR/FILE/plate3.csv"
# )
# your_plate_layout <- "PATH/TO/YOUR/FILE/plate_layout.xlsx"

Run Classification: Yes

final_analysis <- runPvSeroPipeline(
  raw_data = your_raw_data, 
  plate_layout = your_plate_layout, 
  platform = "magpix", 
  location = "ETH", 
  experiment_name = "experiment1", 
  classify = "Yes", 
  algorithm_type = "antibody_model", 
  sens_spec = "maximised"
)

Classification

This is a table containing the classification results (seropositive or seronegative) for each SampleID. In this case, the classification results are stored in the pred_class_max column as we chose the sens_spec = "maximised". If you change it to another type of threshold, then the suffix of that column will change accordingly.

You will also see the relative antibody unit (RAU) values (columns with antigen names), whether the sample passed QC check (QC_total) and the plate that they were run on.

final_analysis[[1]] %>%
  head() %>% 
  kable()

SampleID	Plate	QC_total	EBP	LF005	LF010	LF016	MSP8	PTEX150	PvCSS	RBP2b.P87	pred_class_max
ABC013	plate1	pass	0.0003339	0.0015045	0.0002163	0.0014567	0.0000195	0.0001591	0.0000772	0.0003714	seropositive
ABC097	plate2	pass	0.0004324	0.0015615	0.0001944	0.0013373	0.0000195	0.0001549	0.0000705	0.0009189	seropositive
ABC181	plate3	pass	0.0003822	0.0015832	0.0002144	0.0013711	0.0000195	0.0001582	0.0000710	0.0002070	seropositive
ABC022	plate1	pass	0.0200000	0.0200000	0.0007373	0.0194885	0.0006247	0.0003145	0.0006008	0.0004895	seropositive
ABC106	plate2	pass	0.0057123	0.0193731	0.0007458	0.0195240	0.0006263	0.0003077	0.0006480	0.0126203	seropositive
ABC190	plate3	pass	0.0098260	0.0200000	0.0007400	0.0200000	0.0006020	0.0003171	0.0006555	0.0175253	seropositive

Standard Curve Plot

The standard curve plots are generated from the antibody data from the standards you indicated in your plate layout (e.g. S1-S10) and Median Fluorescent Intensity (MFI) units are displayed in log10-scale. In the case of the PvSeroTaT multi-antigen panel, the antigens will be displayed and in general your standard curves should look relatively linear (only when the y-axis is on logarithmic scale).

final_analysis[[2]]

Bead Counts QC Plot

A summary of the bead counts for each plate well are displayed, with blue indicating there are sufficient beads (≥15) or red when there are not enough. If any of the wells are red, they should be double-checked manually and re-run on a new plate if required.

The function will inform you whether there are “No repeats necessary” or provide a list of samples to be re-run. In the example data, the beads in plate 2 wells A1 and A2 will need to be repeated

final_analysis[[3]] # Plot

final_analysis[[4]] # Samples to repeat 

# A tibble: 2 × 4
  Location SampleID Plate  QC   
  <chr>    <chr>    <chr>  <chr>
1 A1       Blank1   plate2 fail 
2 A2       Blank2   plate2 fail 

Blanks QC Plot

The Median Fluorescent Intensity (MFI) units for each antigen is displayed for your blank samples. In general, each blank sample should have ≤50 MFI for each antigen, if they are higher they should be cross-checked manually.

In the example data, blank samples recorded higher MFI values for LF005 on plate 1 and should be checked to confirm this is expected from the assay.

final_analysis[[5]]

Model Output Plot

The automated data processing in this app allows you to convert your Median Fluorescent Intensity (MFI) data into Relative Antibody Units (RAU) by fitting a 5-parameter logistic function to the standard curve on a per-antigen level. The results from this log-log conversion should look relatively linear for each antigen.

final_analysis[[6]]

$plate1

$plate2

$plate3

Run Classification: No

no_classification_final_analysis <- runPvSeroPipeline(
  raw_data = your_raw_data, 
  plate_layout = your_plate_layout, 
  platform = "magpix", 
  location = "ETH", 
  experiment_name = "experiment1", 
  classify = "No", ########################## key if you do NOT want any classification performed i.e., you do not have PvSeroTaT antigens 
  algorithm_type = "antibody_model", 
  sens_spec = "maximised"
)

MFI and RAU Data

no_classification_final_analysis[[1]]  %>%
  head() %>% 
  kable()

SampleID Location.2 Location Sample Plate QC_total EBP_MFI EBP_Dilution LF005_MFI LF005_Dilution LF010_MFI LF010_Dilution LF016_MFI LF016_Dilution MSP8_MFI MSP8_Dilution PTEX150_MFI PTEX150_Dilution PvCSS_MFI PvCSS_Dilution RBP2b.P87_MFI RBP2b.P87_Dilution ABC013 B1 13(1,B1) Unknown013 plate1 pass 2712.0 0.0003339 1569.0 0.0015045 673.0 0.0002163 327.0 0.0014567 182.0 0.0000195 936.0 0.0001591 223.0 0.0000772 1068.0 0.0003714 ABC014 B2 14(1,B2) Unknown014 plate1 pass 134.0 0.0000216 378.0 0.0004204 117.0 0.0000277 197.0 0.0010331 58.0 0.0000195 122.0 0.0000225 93.0 0.0000298 465.5 0.0001536 ABC015 B3 15(1,B3) Unknown015 plate1 pass 182.0 0.0000232 209.0 0.0002466 208.0 0.0000616 374.0 0.0015985 221.5 0.0000195 293.0 0.0000535 868.0 0.0002235 463.0 0.0001527 ABC016 B4 16(1,B4) Unknown016 plate1 pass 152.0 0.0000222 229.5 0.0002688 101.0 0.0000244 89.0 0.0005994 48.0 0.0000195 109.0 0.0000220 110.0 0.0000383 591.0 0.0001989 ABC017 B5 17(1,B5) Unknown017 plate1 pass 1135.0 0.0001478 236.0 0.0002758 299.0 0.0000950 507.0 0.0019840 209.5 0.0000195 1665.5 0.0002668 266.0 0.0000893 671.0 0.0002277 ABC018 B6 18(1,B6) Unknown018 plate1 pass 174.0 0.0000229 395.0 0.0004370 175.0 0.0000460 78.0 0.0005452 70.0 0.0000195 294.5 0.0000537 92.0 0.0000296 103.0 0.0000238 ABC019 B7 19(1,B7) Unknown019 plate1 pass 421.0 0.0000561 2081.5 0.0019947 529.0 0.0001711 149.0 0.0008557 962.0 0.0002289 241.0 0.0000420 282.0 0.0000936 1441.5 0.0005105 ABC020 B8 20(1,B8) Unknown020 plate1 pass 24.0 0.0000195 49.0 0.0000459 22.0 0.0000195 13.0 0.0000793 15.5 0.0000195 28.0 0.0000195 17.0 0.0000195 14.0 0.0000195 ABC021 B9 21(1,B9) Unknown021 plate1 pass 24.0 0.0000195 45.0 0.0000418 22.0 0.0000195 13.0 0.0000793 16.0 0.0000195 29.0 0.0000195 17.0 0.0000195 13.0 0.0000195 ABC022 B10 22(1,B10) Unknown022 plate1 pass 21464.0 0.0200000 11789.0 0.0200000 2508.5 0.0007373 8001.0 0.0194885 2342.0 0.0006247 1992.0 0.0003145 2927.0 0.0006008 1386.0 0.0004895 ABC023 B11 23(1,B11) Unknown023 plate1 pass 795.0 0.0001063 6135.5 0.0083191 407.0 0.0001314 273.0 0.0012877 535.5 0.0001121 8821.0 0.0017564 998.0 0.0002487 1575.0 0.0005618 ABC024 B12 24(1,B12) Unknown024 plate1 pass 1574.0 0.0001998 348.5 0.0003913 892.0 0.0002826 288.0 0.0013354 306.0 0.0000534 315.0 0.0000587 366.0 0.0001151 590.0 0.0001985 ABC025 C1 25(1,C1) Unknown025 plate1 pass 146.0 0.0000220 408.5 0.0004500 1130.5 0.0003524 83.0 0.0005702 100.0 0.0000195 652.0 0.0001152 130.0 0.0000443 301.0 0.0000937 ABC026 C2 26(1,C2) Unknown026 plate1 pass 1330.5 0.0001711 3036.0 0.0030235 965.0 0.0003042 174.0 0.0009502 79.0 0.0000195 179.0 0.0000303 92.0 0.0000296 1090.0 0.0003794 ABC027 C3 27(1,C3) Unknown027 plate1 pass 358.0 0.0000437 4074.5 0.0043973 335.5 0.0001074 140.0 0.0008203 390.0 0.0000742 8814.0 0.0017540 5627.0 0.0011982 6704.0 0.0051303 ABC028 C4 28(1,C4) Unknown028 plate1 pass 481.0 0.0000657 1870.0 0.0017883 421.0 0.0001360 182.5 0.0009813 1777.5 0.0004609 2465.0 0.0003842 411.0 0.0001261 2390.0 0.0009001 ABC029 C5 29(1,C5) Unknown029 plate1 pass 551.0 0.0000750 1282.0 0.0012423 331.5 0.0001061 165.0 0.0009168 77.0 0.0000195 579.0 0.0001034 229.0 0.0000789 244.0 0.0000729 ABC030 C6 30(1,C6) Unknown030 plate1 pass 943.0 0.0001246 584.5 0.0006156 235.0 0.0000728 135.0 0.0008003 3017.0 0.0008235 4928.0 0.0007896 7730.0 0.0019028 1034.0 0.0003590 ABC031 C7 31(1,C7) Unknown031 plate1 pass 532.5 0.0000726 1533.0 0.0014712 315.0 0.0001005 210.0 0.0010785 2575.5 0.0006931 2779.0 0.0004314 7300.0 0.0017309 2903.0 0.0011432 ABC032 C8 32(1,C8) Unknown032 plate1 pass 768.0 0.0001029 12605.0 0.0200000 409.0 0.0001321 7632.0 0.0193282 9860.0 0.0031020 5612.0 0.0009222 6383.0 0.0014165 13703.0 0.0182981 ABC033 C9 33(1,C9) Unknown033 plate1 pass 130.0 0.0000215 239.5 0.0002795 72.0 0.0000221 64.0 0.0004711 37.0 0.0000195 204.0 0.0000357 49.0 0.0000220 156.0 0.0000377 ABC034 C10 34(1,C10) Unknown034 plate1 pass 40.0 0.0000195 60.0 0.0000664 37.0 0.0000195 25.0 0.0002059 26.5 0.0000195 40.0 0.0000195 27.0 0.0000195 23.5 0.0000195 ABC035 C11 35(1,C11) Unknown035 plate1 pass 167.0 0.0000227 605.0 0.0006344 253.0 0.0000791 329.0 0.0014628 3973.0 0.0011109 803.0 0.0001388 1670.0 0.0003723 1426.5 0.0005048 ABC036 C12 36(1,C12) Unknown036 plate1 pass 1879.0 0.0002355 4562.0 0.0051700 415.0 0.0001340 18287.0 0.0200000 11482.5 0.0037409 4830.5 0.0007715 4217.0 0.0008595 12031.0 0.0182981 ABC037 D1 37(1,D1) Unknown037 plate1 pass 256.0 0.0000281 1507.0 0.0014473 117.0 0.0000277 129.0 0.0007758 168.0 0.0000195 908.0 0.0001549 87.0 0.0000275 488.0 0.0001617 ABC038 D2 38(1,D2) Unknown038 plate1 pass 24.0 0.0000195 49.0 0.0000459 22.0 0.0000195 12.0 0.0000671 15.0 0.0000195 29.0 0.0000195 17.0 0.0000195 14.0 0.0000195 ABC039 D3 39(1,D3) Unknown039 plate1 pass 384.0 0.0000463 1708.0 0.0016343 326.0 0.0001042 1172.5 0.0038643 986.0 0.0002357 299.5 0.0000559 149.0 0.0000528 1041.0 0.0003615 ABC040 D4 40(1,D4) Unknown040 plate1 pass 210.0 0.0000241 512.0 0.0005483 221.0 0.0000679 191.0 0.0010118 89.0 0.0000195 410.0 0.0000754 174.5 0.0000627 203.0 0.0000565 ABC041 D5 41(1,D5) Unknown041 plate1 pass 351.5 0.0000430 312.0 0.0003547 165.5 0.0000435 88.0 0.0005946 66.0 0.0000195 200.0 0.0000337 183.0 0.0000653 205.0 0.0000572 ABC042 D6 42(1,D6) Unknown042 plate1 pass 1278.0 0.0001649 5810.0 0.0077393 275.0 0.0000867 230.5 0.0011484 137.0 0.0000195 1762.0 0.0002809 1241.0 0.0002943 2032.0 0.0007453 ABC043 D7 43(1,D7) Unknown043 plate1 pass 318.0 0.0000381 397.0 0.0004389 130.0 0.0000293 130.5 0.0007820 78.0 0.0000195 1513.5 0.0002446 325.0 0.0001048 903.0 0.0003114 ABC044 D8 44(1,D8) Unknown044 plate1 pass 493.5 0.0000674 2980.5 0.0029584 401.0 0.0001294 5932.5 0.0176633 3536.0 0.0009786 574.0 0.0001026 5389.0 0.0011355 4699.5 0.0023181 ABC045 D9 45(1,D9) Unknown045 plate1 pass 9686.0 0.0016643 873.0 0.0008762 333.0 0.0001066 166.0 0.0009205 791.0 0.0001814 417.5 0.0000766 262.0 0.0000882 1093.0 0.0003805 ABC046 D10 46(1,D10) Unknown046 plate1 pass 777.5 0.0001041 581.0 0.0006123 241.5 0.0000751 173.0 0.0009465 1479.5 0.0003754 1504.0 0.0002432 260.0 0.0000876 899.0 0.0003099 ABC047 D11 47(1,D11) Unknown047 plate1 pass 455.0 0.0000604 416.5 0.0004578 126.0 0.0000288 67.0 0.0004876 48.0 0.0000195 183.0 0.0000307 61.0 0.0000230 400.0 0.0001298 ABC048 D12 48(1,D12) Unknown048 plate1 pass 202.0 0.0000239 1142.0 0.0011165 137.5 0.0000342 176.0 0.0009576 204.5 0.0000195 504.0 0.0000912 459.0 0.0001373 1090.0 0.0003794 ABC049 E1 49(1,E1) Unknown049 plate1 pass 333.5 0.0000395 623.5 0.0006513 368.0 0.0001184 10430.0 0.0200000 2340.0 0.0006241 1650.0 0.0002645 7801.5 0.0019332 4375.0 0.0020540 ABC050 E2 50(1,E2) Unknown050 plate1 pass 561.0 0.0000763 7984.0 0.0126483 441.0 0.0001426 128.0 0.0007717 141.0 0.0000195 2147.0 0.0003372 1502.0 0.0003420 1993.0 0.0007291 ABC051 E3 51(1,E3) Unknown051 plate1 pass 506.5 0.0000691 340.0 0.0003829 152.5 0.0000371 147.0 0.0008479 90.0 0.0000195 2178.0 0.0003417 170.0 0.0000613 600.0 0.0002021 ABC052 E4 52(1,E4) Unknown052 plate1 pass 254.0 0.0000280 367.0 0.0004096 392.5 0.0001266 89.0 0.0005994 142.5 0.0000195 298.0 0.0000557 138.0 0.0000480 280.0 0.0000861 ABC053 E5 53(1,E5) Unknown053 plate1 pass 888.0 0.0001178 1955.0 0.0018705 438.0 0.0001416 173.0 0.0009465 507.0 0.0001046 709.0 0.0001242 2040.0 0.0004386 1009.0 0.0003498 ABC054 E6 54(1,E6) Unknown054 plate1 pass 426.0 0.0000567 1877.0 0.0017951 186.0 0.0000508 9767.0 0.0200000 15945.0 0.0057838 1585.5 0.0002551 1124.0 0.0002726 10073.0 0.0182981 ABC055 E7 55(1,E7) Unknown055 plate1 pass 5733.0 0.0007477 1301.5 0.0012599 1920.5 0.0005747 220.5 0.0011145 4833.0 0.0013763 5329.0 0.0008659 2369.0 0.0004980 2340.0 0.0008778 ABC056 E8 56(1,E8) Unknown056 plate1 pass 19092.0 0.0200000 14422.0 0.0200000 1993.0 0.0005948 12836.5 0.0200000 7426.0 0.0022244 4826.0 0.0007707 3468.5 0.0007051 11930.0 0.0182981 ABC057 E9 57(1,E9) Unknown057 plate1 pass 596.0 0.0000809 15139.0 0.0200000 371.5 0.0001196 229.0 0.0011433 535.5 0.0001121 7928.5 0.0014788 1414.5 0.0003261 14032.0 0.0182981 ABC058 E10 58(1,E10) Unknown058 plate1 pass 1028.0 0.0001349 2646.0 0.0025813 1486.0 0.0004536 292.0 0.0013480 9357.0 0.0029132 3736.0 0.0005820 14370.5 0.0135476 8009.0 0.0124907 ABC059 E11 59(1,E11) Unknown059 plate1 pass 512.0 0.0000699 4735.0 0.0054689 3408.0 0.0009890 127.5 0.0007696 191.0 0.0000195 11567.0 0.0030172 2923.5 0.0006002 4401.0 0.0020741 ABC060 E12 60(1,E12) Unknown060 plate1 pass 883.0 0.0001172 1953.0 0.0018686 755.0 0.0002414 586.0 0.0022066 122.0 0.0000195 425.5 0.0000780 121.0 0.0000411 830.0 0.0002850 ABC061 F1 61(1,F1) Unknown061 plate1 pass 977.0 0.0001287 8719.0 0.0147010 509.0 0.0001646 187.0 0.0009975 1289.0 0.0003211 13891.0 0.0051695 9531.0 0.0028952 12930.0 0.0182981 ABC062 F2 62(1,F2) Unknown062 plate1 pass 791.0 0.0001058 1944.0 0.0018598 465.0 0.0001504 216.5 0.0011009 12307.0 0.0040845 4157.0 0.0006523 760.0 0.0002020 5029.0 0.0026204 ABC063 F3 63(1,F3) Unknown063 plate1 pass 397.0 0.0000494 987.0 0.0009780 924.0 0.0002921 133.0 0.0007922 79.0 0.0000195 2317.5 0.0003623 1080.5 0.0002644 253.0 0.0000762 ABC064 F4 64(1,F4) Unknown064 plate1 pass 1111.0 0.0001450 613.0 0.0006417 357.0 0.0001147 196.0 0.0010296 8496.0 0.0025993 5772.5 0.0009550 13202.0 0.0097890 7914.0 0.0115630 ABC065 F5 65(1,F5) Unknown065 plate1 pass 956.0 0.0001262 2369.0 0.0022864 644.0 0.0002073 389.0 0.0016430 8762.0 0.0026951 6437.0 0.0010988 21113.0 0.0181042 9649.0 0.0182981 ABC066 F6 66(1,F6) Unknown066 plate1 pass 1307.0 0.0001684 20590.0 0.0200000 1335.0 0.0004109 10780.0 0.0200000 12736.0 0.0042688 5493.0 0.0008983 7696.0 0.0018885 25375.5 0.0182981 ABC067 F7 67(1,F7) Unknown067 plate1 pass 433.0 0.0000576 382.0 0.0004243 173.0 0.0000455 105.0 0.0006732 59.0 0.0000195 655.0 0.0001156 367.0 0.0001154 480.0 0.0001588 ABC068 F8 68(1,F8) Unknown068 plate1 pass 161.0 0.0000225 279.0 0.0003210 2524.5 0.0007418 105.5 0.0006755 55.0 0.0000195 681.5 0.0001198 472.5 0.0001405 307.0 0.0000959 ABC069 F9 69(1,F9) Unknown069 plate1 pass 439.0 0.0000584 732.5 0.0007502 397.0 0.0001281 2108.0 0.0069600 9692.0 0.0030385 9513.0 0.0020062 7341.0 0.0017465 8090.0 0.0133753 ABC070 F10 70(1,F10) Unknown070 plate1 pass 1837.0 0.0002306 11406.0 0.0200000 467.5 0.0001512 20011.0 0.0200000 19606.0 0.0078811 5822.0 0.0009653 5127.5 0.0010695 24476.5 0.0182981 ABC071 F11 71(1,F11) Unknown071 plate1 pass 244.0 0.0000275 1665.5 0.0015944 166.0 0.0000437 124.0 0.0007551 154.0 0.0000195 718.0 0.0001256 115.0 0.0000396 224.0 0.0000637 ABC072 F12 72(1,F12) Unknown072 plate1 pass 482.0 0.0000659 3990.5 0.0042734 388.0 0.0001251 3736.0 0.0129192 7436.0 0.0022279 4111.5 0.0006446 2819.0 0.0005806 7361.0 0.0071706 ABC073 G1 73(1,G1) Unknown073 plate1 pass 656.0 0.0000887 3103.0 0.0031029 505.0 0.0001634 14386.0 0.0200000 2343.0 0.0006250 357.5 0.0000663 1215.0 0.0002895 5503.0 0.0031323 ABC074 G2 74(1,G2) Unknown074 plate1 pass 243.0 0.0000275 568.5 0.0006008 693.0 0.0002224 141.0 0.0008243 79.0 0.0000195 2080.0 0.0003273 1470.0 0.0003362 339.0 0.0001076 ABC075 G3 75(1,G3) Unknown075 plate1 pass 1409.5 0.0001805 1123.0 0.0010995 379.0 0.0001221 153.0 0.0008712 167.5 0.0000195 1553.0 0.0002504 1738.0 0.0003845 1013.0 0.0003513 ABC076 G4 76(1,G4) Unknown076 plate1 pass 467.5 0.0000639 6741.0 0.0095659 238.0 0.0000738 174.0 0.0009502 181.0 0.0000195 1660.5 0.0002661 1087.0 0.0002656 6160.0 0.0040573 ABC077 G5 77(1,G5) Unknown077 plate1 pass 329.0 0.0000391 630.0 0.0006573 196.0 0.0000576 119.0 0.0007341 278.0 0.0000467 2052.5 0.0003233 2221.0 0.0004712 787.0 0.0002695 ABC078 G6 78(1,G6) Unknown078 plate1 pass 1122.5 0.0001463 17810.0 0.0200000 677.0 0.0002175 12254.0 0.0200000 16011.0 0.0058178 3551.0 0.0005519 10222.5 0.0034698 18719.0 0.0182981 ABC079 G7 79(1,G7) Unknown079 plate1 pass 10853.0 0.0021309 817.5 0.0008266 255.0 0.0000798 189.0 0.0010047 2247.5 0.0005971 590.0 0.0001052 258.0 0.0000871 3322.0 0.0013652 ABC080 G8 80(1,G8) Unknown080 plate1 pass 743.5 0.0000998 820.0 0.0008288 313.5 0.0001000 289.0 0.0013385 9351.5 0.0029112 3062.0 0.0004748 676.5 0.0001849 7171.0 0.0064529 ABC081 G9 81(1,G9) Unknown081 plate1 pass 723.0 0.0000972 1255.0 0.0012180 299.0 0.0000950 253.0 0.0012229 95.0 0.0000195 396.0 0.0000729 129.5 0.0000441 712.0 0.0002424 ABC082 G10 82(1,G10) Unknown082 plate1 pass 168.0 0.0000227 684.0 0.0007064 131.0 0.0000294 198.0 0.0010366 135.5 0.0000195 292.0 0.0000533 254.5 0.0000861 392.0 0.0001269 ABC083 G11 83(1,G11) Unknown083 plate1 pass 180.0 0.0000231 276.0 0.0003179 205.0 0.0000606 4646.5 0.0152212 2550.0 0.0006856 724.0 0.0001265 2707.0 0.0005598 2664.5 0.0010267 ABC084 G12 84(1,G12) Unknown084 plate1 pass 249.5 0.0000278 4104.0 0.0044414 250.0 0.0000780 96.0 0.0006323 68.0 0.0000195 960.0 0.0001627 672.0 0.0001839 1020.0 0.0003538 ABC085 H1 85(1,H1) Unknown085 plate1 pass 479.0 0.0000655 190.0 0.0002256 105.0 0.0000248 131.0 0.0007840 66.0 0.0000195 1724.5 0.0002754 108.0 0.0000378 1449.0 0.0005134 ABC086 H2 86(1,H2) Unknown086 plate1 pass 164.0 0.0000226 327.0 0.0003698 234.5 0.0000726 66.0 0.0004821 75.5 0.0000195 260.0 0.0000464 89.0 0.0000291 259.0 0.0000784 ABC087 H3 87(1,H3) Unknown087 plate1 pass 281.5 0.0000333 1794.0 0.0017156 413.0 0.0001334 75.0 0.0005299 469.5 0.0000947 345.0 0.0000641 744.5 0.0001988 802.0 0.0002749 ABC088 H4 88(1,H4) Unknown088 plate1 pass 269.0 0.0000323 1067.0 0.0010494 141.0 0.0000349 7595.0 0.0193135 14464.0 0.0050528 804.5 0.0001390 467.0 0.0001392 7592.0 0.0082711 ABC089 H5 89(1,H5) Unknown089 plate1 pass 3247.0 0.0003991 382.0 0.0004243 470.0 0.0001520 98.0 0.0006415 2319.5 0.0006181 1521.0 0.0002457 1258.0 0.0002975 2009.0 0.0007358 ABC090 H6 90(1,H6) Unknown090 plate1 pass 20593.0 0.0200000 14902.5 0.0200000 2021.0 0.0006025 10184.0 0.0200000 5805.5 0.0016853 4307.5 0.0006782 3073.0 0.0006284 14083.5 0.0182981 ABC091 H7 91(1,H7) Unknown091 plate1 pass 395.0 0.0000492 9914.0 0.0177124 211.0 0.0000626 131.5 0.0007861 325.0 0.0000580 4608.0 0.0007312 1073.5 0.0002631 3021.0 0.0012034 ABC092 H8 92(1,H8) Unknown092 plate1 pass 904.5 0.0001198 1871.0 0.0017893 793.0 0.0002529 172.0 0.0009429 6106.0 0.0017829 1303.0 0.0002138 6595.5 0.0014839 2093.0 0.0007710 ABC093 H9 93(1,H9) Unknown093 plate1 pass 323.0 0.0000385 2007.5 0.0019218 1950.0 0.0005829 106.0 0.0006777 191.0 0.0000195 7377.5 0.0013278 1699.0 0.0003775 2411.0 0.0009095 ABC094 H10 94(1,H10) Unknown094 plate1 pass 706.0 0.0000950 2087.0 0.0020002 791.0 0.0002523 169.0 0.0009317 66.0 0.0000195 261.0 0.0000465 65.0 0.0000234 990.0 0.0003429 ABC095 H11 95(1,H11) Unknown095 plate1 pass 323.0 0.0000385 4950.0 0.0058606 264.0 0.0000829 107.0 0.0006821 645.0 0.0001415 8856.0 0.0017682 5330.0 0.0011204 7092.0 0.0061911 ABC096 H12 96(1,H12) Unknown096 plate1 pass 367.0 0.0000446 1067.0 0.0010494 259.0 0.0000812 101.0 0.0006552 7423.0 0.0022234 1841.5 0.0002925 335.0 0.0001074 1203.0 0.0004211 ABC097 B1 13(1,B1) Unknown097 plate2 pass 2712.0 0.0004324 1569.0 0.0015615 673.0 0.0001944 327.0 0.0013373 182.0 0.0000195 936.0 0.0001549 223.0 0.0000705 2208.0 0.0009189 ABC098 B2 14(1,B2) Unknown098 plate2 pass 134.0 0.0000215 378.0 0.0004445 117.0 0.0000238 197.0 0.0009164 58.0 0.0000195 122.0 0.0000222 93.0 0.0000274 290.0 0.0000850 ABC099 B3 15(1,B3) Unknown099 plate2 pass 182.0 0.0000229 209.0 0.0002515 208.0 0.0000444 374.0 0.0014804 221.5 0.0000195 293.0 0.0000512 868.0 0.0002201 376.0 0.0001154 ABC100 B4 16(1,B4) Unknown100 plate2 pass 152.0 0.0000220 229.5 0.0002764 101.0 0.0000227 89.0 0.0005012 48.0 0.0000195 109.0 0.0000217 110.0 0.0000345 103.0 0.0000239 ABC101 B5 17(1,B5) Unknown101 plate2 pass 1135.0 0.0001620 236.0 0.0002842 299.0 0.0000777 507.0 0.0018733 209.5 0.0000195 1665.5 0.0002609 266.0 0.0000819 650.0 0.0002165 ABC102 B6 18(1,B6) Unknown102 plate2 pass 174.0 0.0000226 395.0 0.0004627 175.0 0.0000348 78.0 0.0004511 70.0 0.0000195 294.5 0.0000515 92.0 0.0000273 293.5 0.0000862 ABC103 B7 19(1,B7) Unknown103 plate2 pass 421.0 0.0000514 2081.5 0.0020304 529.0 0.0001498 149.0 0.0007441 962.0 0.0002139 241.0 0.0000392 282.0 0.0000860 1632.5 0.0006324 ABC104 B8 20(1,B8) Unknown104 plate2 pass 24.0 0.0000195 49.0 0.0000338 22.0 0.0000195 13.0 0.0000597 15.5 0.0000195 28.0 0.0000195 17.0 0.0000195 14.0 0.0000195 ABC105 B9 21(1,B9) Unknown105 plate2 pass 24.0 0.0000195 45.0 0.0000280 22.0 0.0000195 13.0 0.0000597 16.0 0.0000195 29.0 0.0000195 17.0 0.0000195 13.0 0.0000195 ABC106 B10 22(1,B10) Unknown106 plate2 pass 21464.0 0.0057123 11789.0 0.0193731 2508.5 0.0007458 8001.0 0.0195240 2342.0 0.0006263 1992.0 0.0003077 2927.0 0.0006480 9995.5 0.0126203 ABC107 B11 23(1,B11) Unknown107 plate2 pass 795.0 0.0001092 6135.5 0.0073751 407.0 0.0001116 273.0 0.0011681 535.5 0.0000973 8821.0 0.0015565 998.0 0.0002476 7092.0 0.0052612 ABC108 B12 24(1,B12) Unknown108 plate2 pass 1574.0 0.0002334 348.5 0.0004125 892.0 0.0002615 288.0 0.0012157 306.0 0.0000428 315.0 0.0000562 366.0 0.0001070 548.0 0.0001781 ABC109 C1 25(1,C1) Unknown109 plate2 pass 146.0 0.0000218 408.5 0.0004771 1130.5 0.0003337 83.0 0.0004742 100.0 0.0000195 652.0 0.0001116 130.0 0.0000403 443.0 0.0001395 ABC110 C2 26(1,C2) Unknown110 plate2 pass 1330.5 0.0001933 3036.0 0.0029675 965.0 0.0002837 174.0 0.0008356 79.0 0.0000195 179.0 0.0000279 92.0 0.0000273 1092.0 0.0003929 ABC111 C3 27(1,C3) Unknown111 plate2 pass 358.0 0.0000396 4074.5 0.0041467 335.5 0.0000892 140.0 0.0007101 390.0 0.0000616 8814.0 0.0015547 5627.0 0.0012826 7093.0 0.0052626 ABC112 C4 28(1,C4) Unknown112 plate2 pass 481.0 0.0000611 1870.0 0.0018351 421.0 0.0001160 182.5 0.0008658 1777.5 0.0004552 2465.0 0.0003758 411.0 0.0001179 1832.0 0.0007279 ABC113 C5 29(1,C5) Unknown113 plate2 pass 551.0 0.0000731 1282.0 0.0013030 331.5 0.0000879 165.0 0.0008031 77.0 0.0000195 579.0 0.0001000 229.0 0.0000721 244.0 0.0000691 ABC114 C6 30(1,C6) Unknown114 plate2 pass 943.0 0.0001319 584.5 0.0006563 235.0 0.0000564 135.0 0.0006908 3017.0 0.0008320 4928.0 0.0007577 7730.0 0.0018913 5339.0 0.0032075 ABC115 C7 31(1,C7) Unknown115 plate2 pass 532.5 0.0000704 1533.0 0.0015290 315.0 0.0000827 210.0 0.0009610 2575.5 0.0006974 2779.0 0.0004215 7300.0 0.0017563 1029.0 0.0003668 ABC116 C8 32(1,C8) Unknown116 plate2 pass 768.0 0.0001051 12605.0 0.0200000 409.0 0.0001123 7632.0 0.0193657 9860.0 0.0029626 5612.0 0.0008768 6383.0 0.0014873 7201.0 0.0054162 ABC117 C9 33(1,C9) Unknown117 plate2 pass 130.0 0.0000214 239.5 0.0002884 72.0 0.0000195 64.0 0.0003835 37.0 0.0000195 204.0 0.0000319 49.0 0.0000220 156.0 0.0000368 ABC118 C10 34(1,C10) Unknown118 plate2 pass 40.0 0.0000195 60.0 0.0000508 37.0 0.0000195 25.0 0.0001517 26.5 0.0000195 40.0 0.0000195 27.0 0.0000195 23.5 0.0000195 ABC119 C11 35(1,C11) Unknown119 plate2 pass 167.0 0.0000224 605.0 0.0006764 253.0 0.0000617 329.0 0.0013435 3973.0 0.0011236 803.0 0.0001348 1670.0 0.0003865 2812.0 0.0012581 ABC120 C12 36(1,C12) Unknown120 plate2 pass 1879.0 0.0002849 4562.0 0.0047821 415.0 0.0001141 18287.0 0.0200000 11482.5 0.0035037 4830.5 0.0007413 4217.0 0.0009350 6291.0 0.0042299 ABC121 D1 37(1,D1) Unknown121 plate2 pass 256.0 0.0000272 1507.0 0.0015056 117.0 0.0000238 129.0 0.0006675 168.0 0.0000195 908.0 0.0001507 87.0 0.0000268 315.0 0.0000938 ABC122 D2 38(1,D2) Unknown122 plate2 pass 24.0 0.0000195 49.0 0.0000338 22.0 0.0000195 12.0 0.0000483 15.0 0.0000195 29.0 0.0000195 17.0 0.0000195 14.0 0.0000195 ABC123 D3 39(1,D3) Unknown123 plate2 pass 384.0 0.0000439 1708.0 0.0016873 326.0 0.0000862 1172.5 0.0038397 986.0 0.0002208 299.5 0.0000523 149.0 0.0000455 902.0 0.0003150 ABC124 D4 40(1,D4) Unknown124 plate2 pass 210.0 0.0000237 512.0 0.0005840 221.0 0.0000492 191.0 0.0008956 89.0 0.0000195 410.0 0.0000725 174.5 0.0000571 398.0 0.0001232 ABC125 D5 41(1,D5) Unknown125 plate2 pass 351.5 0.0000390 312.0 0.0003721 165.5 0.0000315 88.0 0.0004967 66.0 0.0000195 200.0 0.0000315 183.0 0.0000595 174.0 0.0000406 ABC126 D6 42(1,D6) Unknown126 plate2 pass 1278.0 0.0001848 5810.0 0.0067534 275.0 0.0000702 230.5 0.0010298 137.0 0.0000195 1762.0 0.0002748 1241.0 0.0002983 1903.7 0.0007632 ABC127 D7 43(1,D7) Unknown127 plate2 pass 318.0 0.0000360 397.0 0.0004649 130.0 0.0000246 130.5 0.0006734 78.0 0.0000195 1513.5 0.0002391 325.0 0.0000969 492.1 0.0001574 ABC128 D8 44(1,D8) Unknown128 plate2 pass 493.5 0.0000648 2980.5 0.0029098 401.0 0.0001097 5932.5 0.0178504 3536.0 0.0009903 574.0 0.0000993 5389.0 0.0012210 4699.5 0.0026207 ABC129 D9 45(1,D9) Unknown129 plate2 pass 9686.0 0.0019673 873.0 0.0009308 333.0 0.0000884 166.0 0.0008068 791.0 0.0001657 417.5 0.0000737 262.0 0.0000808 653.0 0.0002176 ABC130 D10 46(1,D10) Unknown130 plate2 pass 777.5 0.0001066 581.0 0.0006528 241.5 0.0000583 173.0 0.0008320 1479.5 0.0003657 1504.0 0.0002378 260.0 0.0000803 439.0 0.0001380 ABC131 D11 47(1,D11) Unknown131 plate2 pass 455.0 0.0000576 416.5 0.0004855 126.0 0.0000243 67.0 0.0003984 48.0 0.0000195 183.0 0.0000283 61.0 0.0000229 143.0 0.0000332 ABC132 D12 48(1,D12) Unknown132 plate2 pass 202.0 0.0000235 1142.0 0.0011766 137.5 0.0000251 176.0 0.0008427 204.5 0.0000195 504.0 0.0000880 459.0 0.0001293 210.0 0.0000562 ABC133 E1 49(1,E1) Unknown133 plate2 pass 333.5 0.0000374 623.5 0.0006945 368.0 0.0000994 10430.0 0.0200000 2340.0 0.0006257 1650.0 0.0002587 7801.5 0.0019143 4375.0 0.0023496 ABC134 E2 50(1,E2) Unknown134 plate2 pass 561.0 0.0000745 7984.0 0.0108562 441.0 0.0001223 128.0 0.0006636 141.0 0.0000195 2147.0 0.0003299 1502.0 0.0003520 431.0 0.0001351 ABC135 E3 51(1,E3) Unknown135 plate2 pass 506.5 0.0000666 340.0 0.0004032 152.5 0.0000283 147.0 0.0007366 90.0 0.0000195 2178.0 0.0003344 170.0 0.0000540 299.0 0.0000882 ABC136 E4 52(1,E4) Unknown136 plate2 pass 254.0 0.0000271 367.0 0.0004327 392.5 0.0001071 89.0 0.0005012 142.5 0.0000195 298.0 0.0000520 138.0 0.0000421 323.5 0.0000968 ABC137 E5 53(1,E5) Unknown137 plate2 pass 888.0 0.0001234 1955.0 0.0019132 438.0 0.0001213 173.0 0.0008320 507.0 0.0000901 709.0 0.0001204 2040.0 0.0004624 829.0 0.0002859 ABC138 E6 54(1,E6) Unknown138 plate2 pass 426.0 0.0000520 1877.0 0.0018415 186.0 0.0000367 9767.0 0.0200000 15945.0 0.0051486 1585.5 0.0002495 1124.0 0.0002740 10073.0 0.0128954 ABC139 E7 55(1,E7) Unknown139 plate2 pass 5733.0 0.0010363 1301.5 0.0013205 1920.5 0.0005698 220.5 0.0009965 4833.0 0.0013863 5329.0 0.0008266 2369.0 0.0005305 3718.0 0.0018499 ABC140 E8 56(1,E8) Unknown140 plate2 pass 19092.0 0.0048248 14422.0 0.0200000 1993.0 0.0005914 12836.5 0.0200000 7426.0 0.0021861 4826.0 0.0007405 3468.5 0.0007655 14663.0 0.0180148 ABC141 E9 57(1,E9) Unknown141 plate2 pass 596.0 0.0000796 15139.0 0.0200000 371.5 0.0001005 229.0 0.0010249 535.5 0.0000973 7928.5 0.0013433 1414.5 0.0003340 9105.0 0.0089819 ABC142 E10 58(1,E10) Unknown142 plate2 pass 1028.0 0.0001451 2646.0 0.0025715 1486.0 0.0004402 292.0 0.0012283 9357.0 0.0027992 3736.0 0.0005653 14370.5 0.0052269 7769.5 0.0062936 ABC143 E11 59(1,E11) Unknown143 plate2 pass 512.0 0.0000674 4735.0 0.0050233 3408.0 0.0010202 127.5 0.0006616 191.0 0.0000195 11567.0 0.0023833 2923.5 0.0006473 2401.0 0.0010228 ABC144 E12 60(1,E12) Unknown144 plate2 pass 883.0 0.0001226 1953.0 0.0019114 755.0 0.0002197 586.0 0.0021024 122.0 0.0000195 425.5 0.0000751 121.0 0.0000383 398.0 0.0001232 ABC145 F1 61(1,F1) Unknown145 plate2 pass 977.0 0.0001371 8719.0 0.0124333 509.0 0.0001435 187.0 0.0008816 1289.0 0.0003091 13891.0 0.0033940 9531.0 0.0025298 6039.5 0.0039405 ABC146 F2 62(1,F2) Unknown146 plate2 pass 791.0 0.0001086 1944.0 0.0019031 465.0 0.0001298 216.5 0.0009830 12307.0 0.0037882 4157.0 0.0006313 760.0 0.0001968 3907.0 0.0019872 ABC147 F3 63(1,F3) Unknown147 plate2 pass 397.0 0.0000453 987.0 0.0010357 924.0 0.0002713 133.0 0.0006831 79.0 0.0000195 2317.5 0.0003545 1080.5 0.0002649 253.0 0.0000722 ABC148 F4 64(1,F4) Unknown148 plate2 pass 1111.0 0.0001582 613.0 0.0006843 357.0 0.0000959 196.0 0.0009130 8496.0 0.0025233 5772.5 0.0009058 13202.0 0.0043817 914.0 0.0003199 ABC149 F5 65(1,F5) Unknown149 plate2 pass 956.0 0.0001339 2369.0 0.0023019 644.0 0.0001855 389.0 0.0015255 8762.0 0.0026080 6437.0 0.0010306 21113.0 0.0112485 7649.0 0.0060972 ABC150 F6 66(1,F6) Unknown150 plate2 pass 1307.0 0.0001895 20590.0 0.0200000 1335.0 0.0003951 10780.0 0.0200000 12736.0 0.0039391 5493.0 0.0008555 7696.0 0.0018804 9375.5 0.0107387 ABC151 F7 67(1,F7) Unknown151 plate2 pass 433.0 0.0000529 382.0 0.0004488 173.0 0.0000345 105.0 0.0005703 59.0 0.0000195 655.0 0.0001120 367.0 0.0001073 599.0 0.0001972 ABC152 F8 68(1,F8) Unknown152 plate2 pass 161.0 0.0000223 279.0 0.0003346 2524.5 0.0007506 105.5 0.0005724 55.0 0.0000195 681.5 0.0001162 472.5 0.0001324 491.0 0.0001570 ABC153 F9 69(1,F9) Unknown153 plate2 pass 439.0 0.0000555 732.5 0.0007992 397.0 0.0001085 2108.0 0.0071563 9692.0 0.0029078 9513.0 0.0017380 7341.0 0.0017689 8115.0 0.0068920 ABC154 F10 70(1,F10) Unknown154 plate2 pass 1837.0 0.0002777 11406.0 0.0188821 467.5 0.0001306 20011.0 0.0200000 19606.0 0.0067772 5822.0 0.0009148 5127.5 0.0011547 9476.5 0.0110121 ABC155 F11 71(1,F11) Unknown155 plate2 pass 244.0 0.0000267 1665.5 0.0016488 166.0 0.0000316 124.0 0.0006478 154.0 0.0000195 718.0 0.0001218 115.0 0.0000355 224.0 0.0000606 ABC156 F12 72(1,F12) Unknown156 plate2 pass 482.0 0.0000631 3990.5 0.0040432 388.0 0.0001057 3736.0 0.0132765 7436.0 0.0021893 4111.5 0.0006241 2819.0 0.0006250 2361.0 0.0010009 ABC157 G1 73(1,G1) Unknown157 plate2 pass 656.0 0.0000884 3103.0 0.0030377 505.0 0.0001423 14386.0 0.0200000 2343.0 0.0006266 357.5 0.0000636 1215.0 0.0002929 803.0 0.0002757 ABC158 G2 74(1,G2) Unknown158 plate2 pass 243.0 0.0000266 568.5 0.0006405 693.0 0.0002006 141.0 0.0007139 79.0 0.0000195 2080.0 0.0003203 1470.0 0.0003455 995.0 0.0003528 ABC159 G3 75(1,G3) Unknown159 plate2 pass 1409.5 0.0002062 1123.0 0.0011594 379.0 0.0001028 153.0 0.0007590 167.5 0.0000195 1553.0 0.0002448 1738.0 0.0004004 480.5 0.0001532 ABC160 G4 76(1,G4) Unknown160 plate2 pass 467.5 0.0000593 6741.0 0.0084241 238.0 0.0000573 174.0 0.0008356 181.0 0.0000195 1660.5 0.0002602 1087.0 0.0002663 904.5 0.0003160 ABC161 G5 77(1,G5) Unknown161 plate2 pass 329.0 0.0000370 630.0 0.0007008 196.0 0.0000400 119.0 0.0006278 278.0 0.0000195 2052.5 0.0003164 2221.0 0.0004998 321.0 0.0000959 ABC162 G6 78(1,G6) Unknown162 plate2 pass 1122.5 0.0001600 17810.0 0.0200000 677.0 0.0001957 12254.0 0.0200000 16011.0 0.0051753 3551.0 0.0005369 10222.5 0.0028127 18719.0 0.0185045 ABC163 G7 79(1,G7) Unknown163 plate2 pass 10853.0 0.0022714 817.5 0.0008792 255.0 0.0000623 189.0 0.0008886 2247.5 0.0005976 590.0 0.0001018 258.0 0.0000798 479.0 0.0001526 ABC164 G8 80(1,G8) Unknown164 plate2 pass 743.5 0.0001015 820.0 0.0008815 313.5 0.0000823 289.0 0.0012189 9351.5 0.0027974 3062.0 0.0004632 676.5 0.0001786 771.0 0.0002631 ABC165 G9 81(1,G9) Unknown165 plate2 pass 723.0 0.0000984 1255.0 0.0012786 299.0 0.0000777 253.0 0.0011037 95.0 0.0000195 396.0 0.0000701 129.5 0.0000402 803.0 0.0002757 ABC166 G10 82(1,G10) Unknown166 plate2 pass 168.0 0.0000225 684.0 0.0007530 131.0 0.0000247 198.0 0.0009199 135.5 0.0000195 292.0 0.0000510 254.5 0.0000789 329.0 0.0000987 ABC167 G11 83(1,G11) Unknown167 plate2 pass 180.0 0.0000228 276.0 0.0003311 205.0 0.0000438 4646.5 0.0155910 2550.0 0.0006896 724.0 0.0001227 2707.0 0.0006013 2664.5 0.0011714 ABC168 G12 84(1,G12) Unknown168 plate2 pass 249.5 0.0000269 4104.0 0.0041835 250.0 0.0000608 96.0 0.0005319 68.0 0.0000195 960.0 0.0001585 672.0 0.0001776 702.0 0.0002364 ABC169 H1 85(1,H1) Unknown169 plate2 pass 479.0 0.0000608 190.0 0.0002280 105.0 0.0000230 131.0 0.0006753 66.0 0.0000195 1724.5 0.0002694 108.0 0.0000327 809.0 0.0002780 ABC170 H2 86(1,H2) Unknown170 plate2 pass 164.0 0.0000224 327.0 0.0003888 234.5 0.0000544 66.0 0.0003935 75.5 0.0000195 260.0 0.0000444 89.0 0.0000270 259.0 0.0000743 ABC171 H3 87(1,H3) Unknown171 plate2 pass 281.5 0.0000299 1794.0 0.0017656 413.0 0.0001135 75.0 0.0004370 469.5 0.0000807 345.0 0.0000614 744.5 0.0001935 388.0 0.0001197 ABC172 H4 88(1,H4) Unknown172 plate2 pass 269.0 0.0000278 1067.0 0.0011087 141.0 0.0000271 7595.0 0.0193512 14464.0 0.0045710 804.5 0.0001351 467.0 0.0001311 7592.0 0.0060064 ABC173 H5 89(1,H5) Unknown173 plate2 pass 3247.0 0.0005318 382.0 0.0004488 470.0 0.0001314 98.0 0.0005405 2319.5 0.0006195 1521.0 0.0002402 1258.0 0.0003018 1904.5 0.0007636 ABC174 H6 90(1,H6) Unknown174 plate2 pass 20593.0 0.0053772 14902.5 0.0200000 2021.0 0.0005998 10184.0 0.0200000 5805.5 0.0016843 4307.5 0.0006554 3073.0 0.0006794 9083.5 0.0089291 ABC175 H7 91(1,H7) Unknown175 plate2 pass 395.0 0.0000451 9914.0 0.0153439 211.0 0.0000451 131.5 0.0006773 325.0 0.0000469 4608.0 0.0007043 1073.5 0.0002634 6791.0 0.0048524 ABC176 H8 92(1,H8) Unknown176 plate2 pass 904.5 0.0001259 1871.0 0.0018360 793.0 0.0002313 172.0 0.0008284 6106.0 0.0017767 1303.0 0.0002088 6595.5 0.0015475 5444.0 0.0033110 ABC177 H9 93(1,H9) Unknown177 plate2 pass 323.0 0.0000365 2007.5 0.0019617 1950.0 0.0005786 106.0 0.0005744 191.0 0.0000195 7377.5 0.0012220 1699.0 0.0003924 1411.0 0.0005310 ABC178 H10 94(1,H10) Unknown178 plate2 pass 706.0 0.0000958 2087.0 0.0020356 791.0 0.0002307 169.0 0.0008176 66.0 0.0000195 261.0 0.0000446 65.0 0.0000232 899.0 0.0003138 ABC179 H11 95(1,H11) Unknown179 plate2 pass 323.0 0.0000365 4950.0 0.0053357 264.0 0.0000668 107.0 0.0005786 645.0 0.0001260 8856.0 0.0015653 5330.0 0.0012059 5201.0 0.0030747 ABC180 H12 96(1,H12) Unknown180 plate2 pass 367.0 0.0000404 1067.0 0.0011087 259.0 0.0000652 101.0 0.0005534 7423.0 0.0021852 1841.5 0.0002861 335.0 0.0000994 2494.0 0.0010743 ABC181 B1 13(1,B1) Unknown181 plate3 pass 2712.0 0.0003822 1569.0 0.0015832 673.0 0.0002144 327.0 0.0013711 182.0 0.0000195 936.0 0.0001582 223.0 0.0000710 593.0 0.0002070 ABC182 B2 14(1,B2) Unknown182 plate3 pass 134.0 0.0000222 378.0 0.0004338 117.0 0.0000273 197.0 0.0009908 58.0 0.0000195 122.0 0.0000223 93.0 0.0000291 128.0 0.0000275 ABC183 B3 15(1,B3) Unknown183 plate3 pass 182.0 0.0000235 209.0 0.0002492 208.0 0.0000608 374.0 0.0015032 221.5 0.0000195 293.0 0.0000524 868.0 0.0002190 348.0 0.0001110 ABC184 B4 16(1,B4) Unknown184 plate3 pass 152.0 0.0000227 229.5 0.0002727 101.0 0.0000242 89.0 0.0006276 48.0 0.0000195 109.0 0.0000218 110.0 0.0000351 180.0 0.0000428 ABC185 B5 17(1,B5) Unknown185 plate3 pass 1135.0 0.0001467 236.0 0.0002800 299.0 0.0000941 507.0 0.0018729 209.5 0.0000195 1665.5 0.0002680 266.0 0.0000822 650.0 0.0002296 ABC186 B6 18(1,B6) Unknown186 plate3 pass 174.0 0.0000233 395.0 0.0004515 175.0 0.0000454 78.0 0.0005844 70.0 0.0000195 294.5 0.0000526 92.0 0.0000276 293.5 0.0000900 ABC187 B7 19(1,B7) Unknown187 plate3 pass 421.0 0.0000515 2081.5 0.0020962 529.0 0.0001695 149.0 0.0008387 962.0 0.0002209 241.0 0.0000399 282.0 0.0000862 532.5 0.0001831 ABC188 B8 20(1,B8) Unknown188 plate3 pass 24.0 0.0000195 49.0 0.0000439 22.0 0.0000195 13.0 0.0002395 15.5 0.0000195 28.0 0.0000195 17.0 0.0000195 14.0 0.0000195 ABC189 B9 21(1,B9) Unknown189 plate3 pass 24.0 0.0000195 45.0 0.0000384 22.0 0.0000195 13.0 0.0002395 16.0 0.0000195 29.0 0.0000195 17.0 0.0000195 13.0 0.0000195 ABC190 B10 22(1,B10) Unknown190 plate3 pass 21464.0 0.0098260 11789.0 0.0200000 2508.5 0.0007400 8001.0 0.0200000 2342.0 0.0006020 1992.0 0.0003171 2927.0 0.0006555 9995.5 0.0175253 ABC191 B11 23(1,B11) Unknown191 plate3 pass 795.0 0.0001019 6135.5 0.0085550 407.0 0.0001302 273.0 0.0012167 535.5 0.0001075 8821.0 0.0018293 998.0 0.0002466 12560.0 0.0184996 ABC192 B12 24(1,B12) Unknown192 plate3 pass 1574.0 0.0002077 348.5 0.0004028 892.0 0.0002802 288.0 0.0012600 306.0 0.0000503 315.0 0.0000576 366.0 0.0001069 2060.0 0.0008380 ABC193 C1 25(1,C1) Unknown193 plate3 pass 146.0 0.0000225 408.5 0.0004655 1130.5 0.0003498 83.0 0.0006043 100.0 0.0000195 652.0 0.0001140 130.0 0.0000410 201.0 0.0000538 ABC194 C2 26(1,C2) Unknown194 plate3 pass 1330.5 0.0001734 3036.0 0.0031647 965.0 0.0003017 174.0 0.0009191 79.0 0.0000195 179.0 0.0000283 92.0 0.0000276 709.0 0.0002531 ABC195 C3 27(1,C3) Unknown195 plate3 pass 358.0 0.0000403 4074.5 0.0045841 335.5 0.0001064 140.0 0.0008089 390.0 0.0000706 8814.0 0.0018269 5627.0 0.0013237 5769.5 0.0035886 ABC196 C4 28(1,C4) Unknown196 plate3 pass 481.0 0.0000602 1870.0 0.0018806 421.0 0.0001348 182.5 0.0009458 1777.5 0.0004446 2465.0 0.0003895 411.0 0.0001176 1845.0 0.0007372 ABC197 C5 29(1,C5) Unknown197 plate3 pass 551.0 0.0000708 1282.0 0.0013072 331.5 0.0001051 165.0 0.0008905 77.0 0.0000195 579.0 0.0001022 229.0 0.0000726 244.0 0.0000711 ABC198 C6 30(1,C6) Unknown198 plate3 pass 943.0 0.0001212 584.5 0.0006421 235.0 0.0000719 135.0 0.0007921 3017.0 0.0007929 4928.0 0.0008163 7730.0 0.0019773 5339.0 0.0031148 ABC199 C7 31(1,C7) Unknown199 plate3 pass 532.5 0.0000685 1533.0 0.0015482 315.0 0.0000995 210.0 0.0010304 2575.5 0.0006677 2779.0 0.0004388 7300.0 0.0018316 4592.5 0.0024246 ABC200 C8 32(1,C8) Unknown200 plate3 pass 768.0 0.0000984 12605.0 0.0200000 409.0 0.0001309 7632.0 0.0199151 9860.0 0.0030063 5612.0 0.0009568 6383.0 0.0015425 9340.5 0.0166097 ABC201 C9 33(1,C9) Unknown201 plate3 pass 130.0 0.0000220 239.5 0.0002840 72.0 0.0000218 64.0 0.0005263 37.0 0.0000195 204.0 0.0000335 49.0 0.0000222 156.0 0.0000361 ABC202 C10 34(1,C10) Unknown202 plate3 pass 40.0 0.0000195 60.0 0.0000633 37.0 0.0000195 25.0 0.0003281 26.5 0.0000195 40.0 0.0000195 27.0 0.0000195 23.5 0.0000195 ABC203 C11 35(1,C11) Unknown203 plate3 pass 167.0 0.0000231 605.0 0.0006622 253.0 0.0000782 329.0 0.0013768 3973.0 0.0010692 803.0 0.0001377 1670.0 0.0003868 1412.0 0.0005442 ABC204 C12 36(1,C12) Unknown204 plate3 pass 1879.0 0.0002520 4562.0 0.0053821 415.0 0.0001328 18287.0 0.0200000 11482.5 0.0036382 4830.5 0.0007972 4217.0 0.0009555 5816.0 0.0036438 ABC205 D1 37(1,D1) Unknown205 plate3 pass 256.0 0.0000280 1507.0 0.0015230 117.0 0.0000273 129.0 0.0007717 168.0 0.0000195 908.0 0.0001539 87.0 0.0000271 315.0 0.0000982 ABC206 D2 38(1,D2) Unknown206 plate3 pass 24.0 0.0000195 49.0 0.0000439 22.0 0.0000195 12.0 0.0002309 15.0 0.0000195 29.0 0.0000195 17.0 0.0000195 14.0 0.0000195 ABC207 D3 39(1,D3) Unknown207 plate3 pass 384.0 0.0000444 1708.0 0.0017193 326.0 0.0001032 1172.5 0.0038512 986.0 0.0002274 299.5 0.0000535 149.0 0.0000477 1562.0 0.0006096 ABC208 D4 40(1,D4) Unknown208 plate3 pass 210.0 0.0000243 512.0 0.0005704 221.0 0.0000670 191.0 0.0009723 89.0 0.0000195 410.0 0.0000741 174.5 0.0000578 293.5 0.0000900 ABC209 D5 41(1,D5) Unknown209 plate3 pass 351.5 0.0000398 312.0 0.0003639 165.5 0.0000429 88.0 0.0006237 66.0 0.0000195 200.0 0.0000319 183.0 0.0000602 218.5 0.0000599 ABC210 D6 42(1,D6) Unknown210 plate3 pass 1278.0 0.0001662 5810.0 0.0079522 275.0 0.0000858 230.5 0.0010920 137.0 0.0000195 1762.0 0.0002825 1241.0 0.0002975 729.5 0.0002613 ABC211 D7 43(1,D7) Unknown211 plate3 pass 318.0 0.0000371 397.0 0.0004536 130.0 0.0000289 130.5 0.0007768 78.0 0.0000195 1513.5 0.0002452 325.0 0.0000969 440.0 0.0001468 ABC212 D8 44(1,D8) Unknown212 plate3 pass 493.5 0.0000636 2980.5 0.0030974 401.0 0.0001282 5932.5 0.0189671 3536.0 0.0009420 574.0 0.0001014 5389.0 0.0012581 4699.5 0.0025149 ABC213 D9 45(1,D9) Unknown213 plate3 pass 9686.0 0.0020775 873.0 0.0009196 333.0 0.0001056 166.0 0.0008937 791.0 0.0001749 417.5 0.0000754 262.0 0.0000811 621.0 0.0002181 ABC214 D10 46(1,D10) Unknown214 plate3 pass 777.5 0.0000996 581.0 0.0006387 241.5 0.0000742 173.0 0.0009160 1479.5 0.0003623 1504.0 0.0002438 260.0 0.0000806 1037.5 0.0003863 ABC215 D11 47(1,D11) Unknown215 plate3 pass 455.0 0.0000572 416.5 0.0004737 126.0 0.0000284 67.0 0.0005391 48.0 0.0000195 183.0 0.0000302 61.0 0.0000231 270.0 0.0000810 ABC216 D12 48(1,D12) Unknown216 plate3 pass 202.0 0.0000241 1142.0 0.0011743 137.5 0.0000336 176.0 0.0009255 204.5 0.0000195 504.0 0.0000899 459.0 0.0001288 897.0 0.0003289 ABC217 E1 49(1,E1) Unknown217 plate3 pass 333.5 0.0000383 623.5 0.0006803 368.0 0.0001173 10430.0 0.0200000 2340.0 0.0006015 1650.0 0.0002657 7801.5 0.0020021 4375.0 0.0022487 ABC218 E2 50(1,E2) Unknown218 plate3 pass 561.0 0.0000721 7984.0 0.0130251 441.0 0.0001413 128.0 0.0007683 141.0 0.0000195 2147.0 0.0003407 1502.0 0.0003518 2119.5 0.0008666 ABC219 E3 51(1,E3) Unknown219 plate3 pass 506.5 0.0000652 340.0 0.0003938 152.5 0.0000366 147.0 0.0008321 90.0 0.0000195 2178.0 0.0003454 170.0 0.0000566 399.0 0.0001308 ABC220 E4 52(1,E4) Unknown220 plate3 pass 254.0 0.0000279 367.0 0.0004223 392.5 0.0001254 89.0 0.0006276 142.5 0.0000195 298.0 0.0000532 138.0 0.0000427 323.5 0.0001015 ABC221 E5 53(1,E5) Unknown221 plate3 pass 888.0 0.0001140 1955.0 0.0019665 438.0 0.0001403 173.0 0.0009160 507.0 0.0001001 709.0 0.0001230 2040.0 0.0004642 1584.0 0.0006194 ABC222 E6 54(1,E6) Unknown222 plate3 pass 426.0 0.0000520 1877.0 0.0018877 186.0 0.0000501 9767.0 0.0200000 15945.0 0.0056933 1585.5 0.0002560 1124.0 0.0002730 10073.0 0.0175989 ABC223 E7 55(1,E7) Unknown223 plate3 pass 5733.0 0.0009724 1301.5 0.0013258 1920.5 0.0005738 220.5 0.0010621 4833.0 0.0013248 5329.0 0.0008972 2369.0 0.0005341 3718.0 0.0017722 ABC224 E8 56(1,E8) Unknown224 plate3 pass 19092.0 0.0073208 14422.0 0.0200000 1993.0 0.0005942 12836.5 0.0200000 7426.0 0.0021467 4826.0 0.0007964 3468.5 0.0007778 8663.0 0.0118795 ABC225 E9 57(1,E9) Unknown225 plate3 pass 596.0 0.0000765 15139.0 0.0200000 371.5 0.0001185 229.0 0.0010876 535.5 0.0001075 7928.5 0.0015422 1414.5 0.0003336 8105.0 0.0082846 ABC226 E10 58(1,E10) Unknown226 plate3 pass 1028.0 0.0001324 2646.0 0.0027065 1486.0 0.0004513 292.0 0.0012714 9357.0 0.0028205 3736.0 0.0005969 14370.5 0.0056660 7769.5 0.0072079 ABC227 E11 59(1,E11) Unknown227 plate3 pass 512.0 0.0000659 4735.0 0.0056910 3408.0 0.0010010 127.5 0.0007666 191.0 0.0000195 11567.0 0.0030729 2923.5 0.0006547 901.0 0.0003305 ABC228 E12 60(1,E12) Unknown228 plate3 pass 883.0 0.0001133 1953.0 0.0019645 755.0 0.0002393 586.0 0.0020929 122.0 0.0000195 425.5 0.0000768 121.0 0.0000390 529.0 0.0001817 ABC229 F1 61(1,F1) Unknown229 plate3 pass 977.0 0.0001257 8719.0 0.0152000 509.0 0.0001632 187.0 0.0009599 1289.0 0.0003100 13891.0 0.0049382 9531.0 0.0026711 5039.5 0.0028202 ABC230 F2 62(1,F2) Unknown230 plate3 pass 791.0 0.0001014 1944.0 0.0019554 465.0 0.0001491 216.5 0.0010501 12307.0 0.0039802 4157.0 0.0006711 760.0 0.0001958 8907.0 0.0134354 ABC231 F3 63(1,F3) Unknown231 plate3 pass 397.0 0.0000456 987.0 0.0010277 924.0 0.0002897 133.0 0.0007853 79.0 0.0000195 2317.5 0.0003667 1080.5 0.0002639 253.0 0.0000745 ABC232 F4 64(1,F4) Unknown232 plate3 pass 1111.0 0.0001435 613.0 0.0006700 357.0 0.0001137 196.0 0.0009877 8496.0 0.0025127 5772.5 0.0009915 13202.0 0.0047167 7914.0 0.0076416 ABC233 F5 65(1,F5) Unknown233 plate3 pass 956.0 0.0001229 2369.0 0.0024001 644.0 0.0002054 389.0 0.0015451 8762.0 0.0026065 6437.0 0.0011435 21113.0 0.0114948 4649.0 0.0024719 ABC234 F6 66(1,F6) Unknown234 plate3 pass 1307.0 0.0001702 20590.0 0.0200000 1335.0 0.0004084 10780.0 0.0200000 12736.0 0.0041643 5493.0 0.0009314 7696.0 0.0019655 7375.5 0.0062016 ABC235 F7 67(1,F7) Unknown235 plate3 pass 433.0 0.0000528 382.0 0.0004380 173.0 0.0000449 105.0 0.0006872 59.0 0.0000195 655.0 0.0001144 367.0 0.0001072 599.0 0.0002094 ABC236 F8 68(1,F8) Unknown236 plate3 pass 161.0 0.0000229 279.0 0.0003280 2524.5 0.0007446 105.5 0.0006891 55.0 0.0000195 681.5 0.0001186 472.5 0.0001320 491.0 0.0001668 ABC237 F9 69(1,F9) Unknown237 plate3 pass 439.0 0.0000553 732.5 0.0007856 397.0 0.0001269 2108.0 0.0075580 9692.0 0.0029437 9513.0 0.0020840 7341.0 0.0018452 6115.0 0.0040204 ABC238 F10 70(1,F10) Unknown238 plate3 pass 1837.0 0.0002458 11406.0 0.0200000 467.5 0.0001499 20011.0 0.0200000 19606.0 0.0078560 5822.0 0.0010024 5127.5 0.0011877 5476.5 0.0032592 ABC239 F11 71(1,F11) Unknown239 plate3 pass 244.0 0.0000275 1665.5 0.0016775 166.0 0.0000430 124.0 0.0007545 154.0 0.0000195 718.0 0.0001244 115.0 0.0000377 224.0 0.0000618 ABC240 F12 72(1,F12) Unknown240 plate3 pass 482.0 0.0000603 3990.5 0.0044563 388.0 0.0001240 3736.0 0.0141769 7436.0 0.0021501 4111.5 0.0006629 2819.0 0.0006317 3361.0 0.0015428 ABC241 G1 73(1,G1) Unknown241 plate3 pass 656.0 0.0000841 3103.0 0.0032470 505.0 0.0001619 14386.0 0.0200000 2343.0 0.0006023 357.5 0.0000651 1215.0 0.0002920 2503.0 0.0010582 ABC242 G2 74(1,G2) Unknown242 plate3 pass 243.0 0.0000275 568.5 0.0006264 693.0 0.0002205 141.0 0.0008122 79.0 0.0000195 2080.0 0.0003305 1470.0 0.0003451 1295.0 0.0004941 ABC243 G3 75(1,G3) Unknown243 plate3 pass 1409.5 0.0001844 1123.0 0.0011563 379.0 0.0001210 153.0 0.0008518 167.5 0.0000195 1553.0 0.0002511 1738.0 0.0004009 1280.5 0.0004879 ABC244 G4 76(1,G4) Unknown244 plate3 pass 467.5 0.0000586 6741.0 0.0098601 238.0 0.0000730 174.0 0.0009191 181.0 0.0000195 1660.5 0.0002672 1087.0 0.0002653 1804.5 0.0007186 ABC245 G5 77(1,G5) Unknown245 plate3 pass 329.0 0.0000380 630.0 0.0006866 196.0 0.0000568 119.0 0.0007371 278.0 0.0000437 2052.5 0.0003263 2221.0 0.0005025 223.0 0.0000615 ABC246 G6 78(1,G6) Unknown246 plate3 pass 1122.5 0.0001451 17810.0 0.0200000 677.0 0.0002156 12254.0 0.0200000 16011.0 0.0057279 3551.0 0.0005652 10222.5 0.0029805 3142.0 0.0014107 ABC247 G7 79(1,G7) Unknown247 plate3 pass 10853.0 0.0024930 817.5 0.0008669 255.0 0.0000789 189.0 0.0009661 2247.5 0.0005755 590.0 0.0001040 258.0 0.0000801 1023.0 0.0003804 ABC248 G8 80(1,G8) Unknown248 plate3 pass 743.5 0.0000952 820.0 0.0008693 313.5 0.0000990 289.0 0.0012628 9351.5 0.0028185 3062.0 0.0004842 676.5 0.0001777 873.0 0.0003191 ABC249 G9 81(1,G9) Unknown249 plate3 pass 723.0 0.0000926 1255.0 0.0012815 299.0 0.0000941 253.0 0.0011585 95.0 0.0000195 396.0 0.0000717 129.5 0.0000409 203.0 0.0000545 ABC250 G10 82(1,G10) Unknown250 plate3 pass 168.0 0.0000231 684.0 0.0007389 131.0 0.0000291 198.0 0.0009938 135.5 0.0000195 292.0 0.0000522 254.5 0.0000792 223.4 0.0000616 ABC251 G11 83(1,G11) Unknown251 plate3 pass 180.0 0.0000234 276.0 0.0003247 205.0 0.0000598 4646.5 0.0168749 2550.0 0.0006605 724.0 0.0001254 2707.0 0.0006071 664.5 0.0002354 ABC252 G12 84(1,G12) Unknown252 plate3 pass 249.5 0.0000277 4104.0 0.0046296 250.0 0.0000772 96.0 0.0006541 68.0 0.0000195 960.0 0.0001619 672.0 0.0001767 563.0 0.0001952 ABC253 H1 85(1,H1) Unknown253 plate3 pass 479.0 0.0000600 190.0 0.0002270 105.0 0.0000245 131.0 0.0007785 66.0 0.0000195 1724.5 0.0002768 108.0 0.0000348 406.0 0.0001335 ABC254 H2 86(1,H2) Unknown254 plate3 pass 164.0 0.0000230 327.0 0.0003800 234.5 0.0000718 66.0 0.0005349 75.5 0.0000195 260.0 0.0000454 89.0 0.0000273 259.0 0.0000768 ABC255 H3 87(1,H3) Unknown255 plate3 pass 281.5 0.0000328 1794.0 0.0018046 413.0 0.0001322 75.0 0.0005723 469.5 0.0000906 345.0 0.0000629 744.5 0.0001925 530.5 0.0001823 ABC256 H4 88(1,H4) Unknown256 plate3 pass 269.0 0.0000320 1067.0 0.0011033 141.0 0.0000343 7595.0 0.0198877 14464.0 0.0049520 804.5 0.0001379 467.0 0.0001307 7592.0 0.0067261 ABC257 H5 89(1,H5) Unknown257 plate3 pass 3247.0 0.0004729 382.0 0.0004380 470.0 0.0001507 98.0 0.0006615 2319.5 0.0005957 1521.0 0.0002464 1258.0 0.0003010 2304.5 0.0009573 ABC258 H6 90(1,H6) Unknown258 plate3 pass 20593.0 0.0088057 14902.5 0.0200000 2021.0 0.0006021 10184.0 0.0200000 5805.5 0.0016233 4307.5 0.0006985 3073.0 0.0006880 14083.5 0.0184996 ABC259 H7 91(1,H7) Unknown259 plate3 pass 395.0 0.0000454 9914.0 0.0183875 211.0 0.0000618 131.5 0.0007802 325.0 0.0000548 4608.0 0.0007545 1073.5 0.0002624 2791.0 0.0012115 ABC260 H8 92(1,H8) Unknown260 plate3 pass 904.5 0.0001161 1871.0 0.0018816 793.0 0.0002507 172.0 0.0009128 6106.0 0.0017178 1303.0 0.0002137 6595.5 0.0016070 744.0 0.0002671 ABC261 H9 93(1,H9) Unknown261 plate3 pass 323.0 0.0000375 2007.5 0.0020201 1950.0 0.0005821 106.0 0.0006909 191.0 0.0000195 7377.5 0.0013845 1699.0 0.0003928 2411.0 0.0010110 ABC262 H10 94(1,H10) Unknown262 plate3 pass 706.0 0.0000904 2087.0 0.0021019 791.0 0.0002501 169.0 0.0009033 66.0 0.0000195 261.0 0.0000456 65.0 0.0000234 412.0 0.0001359 ABC263 H11 95(1,H11) Unknown263 plate3 pass 323.0 0.0000375 4950.0 0.0060963 264.0 0.0000820 107.0 0.0006945 645.0 0.0001361 8856.0 0.0018414 5330.0 0.0012421 2381.0 0.0009958 ABC264 H12 96(1,H12) Unknown264 plate3 pass 367.0 0.0000429 1067.0 0.0011033 259.0 0.0000803 101.0 0.0006726 7423.0 0.0021457 1841.5 0.0002944 335.0 0.0000994 1494.0 0.0005798

SampleID Plate QC_total EBP_MFI EBP_Dilution LF005_MFI LF005_Dilution LF010_MFI LF010_Dilution LF016_MFI LF016_Dilution MSP8_MFI MSP8_Dilution PTEX150_MFI PTEX150_Dilution PvCSS_MFI PvCSS_Dilution RBP2b.P87_MFI RBP2b.P87_Dilution ABC013 plate1 pass 2712.0 0.0003339 1569.0 0.0015045 673.0 0.0002163 327.0 0.0014567 182.0 0.0000195 936.0 0.0001591 223.0 0.0000772 1068.0 0.0003714 ABC014 plate1 pass 134.0 0.0000216 378.0 0.0004204 117.0 0.0000277 197.0 0.0010331 58.0 0.0000195 122.0 0.0000225 93.0 0.0000298 465.5 0.0001536 ABC015 plate1 pass 182.0 0.0000232 209.0 0.0002466 208.0 0.0000616 374.0 0.0015985 221.5 0.0000195 293.0 0.0000535 868.0 0.0002235 463.0 0.0001527 ABC016 plate1 pass 152.0 0.0000222 229.5 0.0002688 101.0 0.0000244 89.0 0.0005994 48.0 0.0000195 109.0 0.0000220 110.0 0.0000383 591.0 0.0001989 ABC017 plate1 pass 1135.0 0.0001478 236.0 0.0002758 299.0 0.0000950 507.0 0.0019840 209.5 0.0000195 1665.5 0.0002668 266.0 0.0000893 671.0 0.0002277 ABC018 plate1 pass 174.0 0.0000229 395.0 0.0004370 175.0 0.0000460 78.0 0.0005452 70.0 0.0000195 294.5 0.0000537 92.0 0.0000296 103.0 0.0000238 ABC019 plate1 pass 421.0 0.0000561 2081.5 0.0019947 529.0 0.0001711 149.0 0.0008557 962.0 0.0002289 241.0 0.0000420 282.0 0.0000936 1441.5 0.0005105 ABC020 plate1 pass 24.0 0.0000195 49.0 0.0000459 22.0 0.0000195 13.0 0.0000793 15.5 0.0000195 28.0 0.0000195 17.0 0.0000195 14.0 0.0000195 ABC021 plate1 pass 24.0 0.0000195 45.0 0.0000418 22.0 0.0000195 13.0 0.0000793 16.0 0.0000195 29.0 0.0000195 17.0 0.0000195 13.0 0.0000195 ABC022 plate1 pass 21464.0 0.0200000 11789.0 0.0200000 2508.5 0.0007373 8001.0 0.0194885 2342.0 0.0006247 1992.0 0.0003145 2927.0 0.0006008 1386.0 0.0004895 ABC023 plate1 pass 795.0 0.0001063 6135.5 0.0083191 407.0 0.0001314 273.0 0.0012877 535.5 0.0001121 8821.0 0.0017564 998.0 0.0002487 1575.0 0.0005618 ABC024 plate1 pass 1574.0 0.0001998 348.5 0.0003913 892.0 0.0002826 288.0 0.0013354 306.0 0.0000534 315.0 0.0000587 366.0 0.0001151 590.0 0.0001985 ABC025 plate1 pass 146.0 0.0000220 408.5 0.0004500 1130.5 0.0003524 83.0 0.0005702 100.0 0.0000195 652.0 0.0001152 130.0 0.0000443 301.0 0.0000937 ABC026 plate1 pass 1330.5 0.0001711 3036.0 0.0030235 965.0 0.0003042 174.0 0.0009502 79.0 0.0000195 179.0 0.0000303 92.0 0.0000296 1090.0 0.0003794 ABC027 plate1 pass 358.0 0.0000437 4074.5 0.0043973 335.5 0.0001074 140.0 0.0008203 390.0 0.0000742 8814.0 0.0017540 5627.0 0.0011982 6704.0 0.0051303 ABC028 plate1 pass 481.0 0.0000657 1870.0 0.0017883 421.0 0.0001360 182.5 0.0009813 1777.5 0.0004609 2465.0 0.0003842 411.0 0.0001261 2390.0 0.0009001 ABC029 plate1 pass 551.0 0.0000750 1282.0 0.0012423 331.5 0.0001061 165.0 0.0009168 77.0 0.0000195 579.0 0.0001034 229.0 0.0000789 244.0 0.0000729 ABC030 plate1 pass 943.0 0.0001246 584.5 0.0006156 235.0 0.0000728 135.0 0.0008003 3017.0 0.0008235 4928.0 0.0007896 7730.0 0.0019028 1034.0 0.0003590 ABC031 plate1 pass 532.5 0.0000726 1533.0 0.0014712 315.0 0.0001005 210.0 0.0010785 2575.5 0.0006931 2779.0 0.0004314 7300.0 0.0017309 2903.0 0.0011432 ABC032 plate1 pass 768.0 0.0001029 12605.0 0.0200000 409.0 0.0001321 7632.0 0.0193282 9860.0 0.0031020 5612.0 0.0009222 6383.0 0.0014165 13703.0 0.0182981 ABC033 plate1 pass 130.0 0.0000215 239.5 0.0002795 72.0 0.0000221 64.0 0.0004711 37.0 0.0000195 204.0 0.0000357 49.0 0.0000220 156.0 0.0000377 ABC034 plate1 pass 40.0 0.0000195 60.0 0.0000664 37.0 0.0000195 25.0 0.0002059 26.5 0.0000195 40.0 0.0000195 27.0 0.0000195 23.5 0.0000195 ABC035 plate1 pass 167.0 0.0000227 605.0 0.0006344 253.0 0.0000791 329.0 0.0014628 3973.0 0.0011109 803.0 0.0001388 1670.0 0.0003723 1426.5 0.0005048 ABC036 plate1 pass 1879.0 0.0002355 4562.0 0.0051700 415.0 0.0001340 18287.0 0.0200000 11482.5 0.0037409 4830.5 0.0007715 4217.0 0.0008595 12031.0 0.0182981 ABC037 plate1 pass 256.0 0.0000281 1507.0 0.0014473 117.0 0.0000277 129.0 0.0007758 168.0 0.0000195 908.0 0.0001549 87.0 0.0000275 488.0 0.0001617 ABC038 plate1 pass 24.0 0.0000195 49.0 0.0000459 22.0 0.0000195 12.0 0.0000671 15.0 0.0000195 29.0 0.0000195 17.0 0.0000195 14.0 0.0000195 ABC039 plate1 pass 384.0 0.0000463 1708.0 0.0016343 326.0 0.0001042 1172.5 0.0038643 986.0 0.0002357 299.5 0.0000559 149.0 0.0000528 1041.0 0.0003615 ABC040 plate1 pass 210.0 0.0000241 512.0 0.0005483 221.0 0.0000679 191.0 0.0010118 89.0 0.0000195 410.0 0.0000754 174.5 0.0000627 203.0 0.0000565 ABC041 plate1 pass 351.5 0.0000430 312.0 0.0003547 165.5 0.0000435 88.0 0.0005946 66.0 0.0000195 200.0 0.0000337 183.0 0.0000653 205.0 0.0000572 ABC042 plate1 pass 1278.0 0.0001649 5810.0 0.0077393 275.0 0.0000867 230.5 0.0011484 137.0 0.0000195 1762.0 0.0002809 1241.0 0.0002943 2032.0 0.0007453 ABC043 plate1 pass 318.0 0.0000381 397.0 0.0004389 130.0 0.0000293 130.5 0.0007820 78.0 0.0000195 1513.5 0.0002446 325.0 0.0001048 903.0 0.0003114 ABC044 plate1 pass 493.5 0.0000674 2980.5 0.0029584 401.0 0.0001294 5932.5 0.0176633 3536.0 0.0009786 574.0 0.0001026 5389.0 0.0011355 4699.5 0.0023181 ABC045 plate1 pass 9686.0 0.0016643 873.0 0.0008762 333.0 0.0001066 166.0 0.0009205 791.0 0.0001814 417.5 0.0000766 262.0 0.0000882 1093.0 0.0003805 ABC046 plate1 pass 777.5 0.0001041 581.0 0.0006123 241.5 0.0000751 173.0 0.0009465 1479.5 0.0003754 1504.0 0.0002432 260.0 0.0000876 899.0 0.0003099 ABC047 plate1 pass 455.0 0.0000604 416.5 0.0004578 126.0 0.0000288 67.0 0.0004876 48.0 0.0000195 183.0 0.0000307 61.0 0.0000230 400.0 0.0001298 ABC048 plate1 pass 202.0 0.0000239 1142.0 0.0011165 137.5 0.0000342 176.0 0.0009576 204.5 0.0000195 504.0 0.0000912 459.0 0.0001373 1090.0 0.0003794 ABC049 plate1 pass 333.5 0.0000395 623.5 0.0006513 368.0 0.0001184 10430.0 0.0200000 2340.0 0.0006241 1650.0 0.0002645 7801.5 0.0019332 4375.0 0.0020540 ABC050 plate1 pass 561.0 0.0000763 7984.0 0.0126483 441.0 0.0001426 128.0 0.0007717 141.0 0.0000195 2147.0 0.0003372 1502.0 0.0003420 1993.0 0.0007291 ABC051 plate1 pass 506.5 0.0000691 340.0 0.0003829 152.5 0.0000371 147.0 0.0008479 90.0 0.0000195 2178.0 0.0003417 170.0 0.0000613 600.0 0.0002021 ABC052 plate1 pass 254.0 0.0000280 367.0 0.0004096 392.5 0.0001266 89.0 0.0005994 142.5 0.0000195 298.0 0.0000557 138.0 0.0000480 280.0 0.0000861 ABC053 plate1 pass 888.0 0.0001178 1955.0 0.0018705 438.0 0.0001416 173.0 0.0009465 507.0 0.0001046 709.0 0.0001242 2040.0 0.0004386 1009.0 0.0003498 ABC054 plate1 pass 426.0 0.0000567 1877.0 0.0017951 186.0 0.0000508 9767.0 0.0200000 15945.0 0.0057838 1585.5 0.0002551 1124.0 0.0002726 10073.0 0.0182981 ABC055 plate1 pass 5733.0 0.0007477 1301.5 0.0012599 1920.5 0.0005747 220.5 0.0011145 4833.0 0.0013763 5329.0 0.0008659 2369.0 0.0004980 2340.0 0.0008778 ABC056 plate1 pass 19092.0 0.0200000 14422.0 0.0200000 1993.0 0.0005948 12836.5 0.0200000 7426.0 0.0022244 4826.0 0.0007707 3468.5 0.0007051 11930.0 0.0182981 ABC057 plate1 pass 596.0 0.0000809 15139.0 0.0200000 371.5 0.0001196 229.0 0.0011433 535.5 0.0001121 7928.5 0.0014788 1414.5 0.0003261 14032.0 0.0182981 ABC058 plate1 pass 1028.0 0.0001349 2646.0 0.0025813 1486.0 0.0004536 292.0 0.0013480 9357.0 0.0029132 3736.0 0.0005820 14370.5 0.0135476 8009.0 0.0124907 ABC059 plate1 pass 512.0 0.0000699 4735.0 0.0054689 3408.0 0.0009890 127.5 0.0007696 191.0 0.0000195 11567.0 0.0030172 2923.5 0.0006002 4401.0 0.0020741 ABC060 plate1 pass 883.0 0.0001172 1953.0 0.0018686 755.0 0.0002414 586.0 0.0022066 122.0 0.0000195 425.5 0.0000780 121.0 0.0000411 830.0 0.0002850 ABC061 plate1 pass 977.0 0.0001287 8719.0 0.0147010 509.0 0.0001646 187.0 0.0009975 1289.0 0.0003211 13891.0 0.0051695 9531.0 0.0028952 12930.0 0.0182981 ABC062 plate1 pass 791.0 0.0001058 1944.0 0.0018598 465.0 0.0001504 216.5 0.0011009 12307.0 0.0040845 4157.0 0.0006523 760.0 0.0002020 5029.0 0.0026204 ABC063 plate1 pass 397.0 0.0000494 987.0 0.0009780 924.0 0.0002921 133.0 0.0007922 79.0 0.0000195 2317.5 0.0003623 1080.5 0.0002644 253.0 0.0000762 ABC064 plate1 pass 1111.0 0.0001450 613.0 0.0006417 357.0 0.0001147 196.0 0.0010296 8496.0 0.0025993 5772.5 0.0009550 13202.0 0.0097890 7914.0 0.0115630 ABC065 plate1 pass 956.0 0.0001262 2369.0 0.0022864 644.0 0.0002073 389.0 0.0016430 8762.0 0.0026951 6437.0 0.0010988 21113.0 0.0181042 9649.0 0.0182981 ABC066 plate1 pass 1307.0 0.0001684 20590.0 0.0200000 1335.0 0.0004109 10780.0 0.0200000 12736.0 0.0042688 5493.0 0.0008983 7696.0 0.0018885 25375.5 0.0182981 ABC067 plate1 pass 433.0 0.0000576 382.0 0.0004243 173.0 0.0000455 105.0 0.0006732 59.0 0.0000195 655.0 0.0001156 367.0 0.0001154 480.0 0.0001588 ABC068 plate1 pass 161.0 0.0000225 279.0 0.0003210 2524.5 0.0007418 105.5 0.0006755 55.0 0.0000195 681.5 0.0001198 472.5 0.0001405 307.0 0.0000959 ABC069 plate1 pass 439.0 0.0000584 732.5 0.0007502 397.0 0.0001281 2108.0 0.0069600 9692.0 0.0030385 9513.0 0.0020062 7341.0 0.0017465 8090.0 0.0133753 ABC070 plate1 pass 1837.0 0.0002306 11406.0 0.0200000 467.5 0.0001512 20011.0 0.0200000 19606.0 0.0078811 5822.0 0.0009653 5127.5 0.0010695 24476.5 0.0182981 ABC071 plate1 pass 244.0 0.0000275 1665.5 0.0015944 166.0 0.0000437 124.0 0.0007551 154.0 0.0000195 718.0 0.0001256 115.0 0.0000396 224.0 0.0000637 ABC072 plate1 pass 482.0 0.0000659 3990.5 0.0042734 388.0 0.0001251 3736.0 0.0129192 7436.0 0.0022279 4111.5 0.0006446 2819.0 0.0005806 7361.0 0.0071706 ABC073 plate1 pass 656.0 0.0000887 3103.0 0.0031029 505.0 0.0001634 14386.0 0.0200000 2343.0 0.0006250 357.5 0.0000663 1215.0 0.0002895 5503.0 0.0031323 ABC074 plate1 pass 243.0 0.0000275 568.5 0.0006008 693.0 0.0002224 141.0 0.0008243 79.0 0.0000195 2080.0 0.0003273 1470.0 0.0003362 339.0 0.0001076 ABC075 plate1 pass 1409.5 0.0001805 1123.0 0.0010995 379.0 0.0001221 153.0 0.0008712 167.5 0.0000195 1553.0 0.0002504 1738.0 0.0003845 1013.0 0.0003513 ABC076 plate1 pass 467.5 0.0000639 6741.0 0.0095659 238.0 0.0000738 174.0 0.0009502 181.0 0.0000195 1660.5 0.0002661 1087.0 0.0002656 6160.0 0.0040573 ABC077 plate1 pass 329.0 0.0000391 630.0 0.0006573 196.0 0.0000576 119.0 0.0007341 278.0 0.0000467 2052.5 0.0003233 2221.0 0.0004712 787.0 0.0002695 ABC078 plate1 pass 1122.5 0.0001463 17810.0 0.0200000 677.0 0.0002175 12254.0 0.0200000 16011.0 0.0058178 3551.0 0.0005519 10222.5 0.0034698 18719.0 0.0182981 ABC079 plate1 pass 10853.0 0.0021309 817.5 0.0008266 255.0 0.0000798 189.0 0.0010047 2247.5 0.0005971 590.0 0.0001052 258.0 0.0000871 3322.0 0.0013652 ABC080 plate1 pass 743.5 0.0000998 820.0 0.0008288 313.5 0.0001000 289.0 0.0013385 9351.5 0.0029112 3062.0 0.0004748 676.5 0.0001849 7171.0 0.0064529 ABC081 plate1 pass 723.0 0.0000972 1255.0 0.0012180 299.0 0.0000950 253.0 0.0012229 95.0 0.0000195 396.0 0.0000729 129.5 0.0000441 712.0 0.0002424 ABC082 plate1 pass 168.0 0.0000227 684.0 0.0007064 131.0 0.0000294 198.0 0.0010366 135.5 0.0000195 292.0 0.0000533 254.5 0.0000861 392.0 0.0001269 ABC083 plate1 pass 180.0 0.0000231 276.0 0.0003179 205.0 0.0000606 4646.5 0.0152212 2550.0 0.0006856 724.0 0.0001265 2707.0 0.0005598 2664.5 0.0010267 ABC084 plate1 pass 249.5 0.0000278 4104.0 0.0044414 250.0 0.0000780 96.0 0.0006323 68.0 0.0000195 960.0 0.0001627 672.0 0.0001839 1020.0 0.0003538 ABC085 plate1 pass 479.0 0.0000655 190.0 0.0002256 105.0 0.0000248 131.0 0.0007840 66.0 0.0000195 1724.5 0.0002754 108.0 0.0000378 1449.0 0.0005134 ABC086 plate1 pass 164.0 0.0000226 327.0 0.0003698 234.5 0.0000726 66.0 0.0004821 75.5 0.0000195 260.0 0.0000464 89.0 0.0000291 259.0 0.0000784 ABC087 plate1 pass 281.5 0.0000333 1794.0 0.0017156 413.0 0.0001334 75.0 0.0005299 469.5 0.0000947 345.0 0.0000641 744.5 0.0001988 802.0 0.0002749 ABC088 plate1 pass 269.0 0.0000323 1067.0 0.0010494 141.0 0.0000349 7595.0 0.0193135 14464.0 0.0050528 804.5 0.0001390 467.0 0.0001392 7592.0 0.0082711 ABC089 plate1 pass 3247.0 0.0003991 382.0 0.0004243 470.0 0.0001520 98.0 0.0006415 2319.5 0.0006181 1521.0 0.0002457 1258.0 0.0002975 2009.0 0.0007358 ABC090 plate1 pass 20593.0 0.0200000 14902.5 0.0200000 2021.0 0.0006025 10184.0 0.0200000 5805.5 0.0016853 4307.5 0.0006782 3073.0 0.0006284 14083.5 0.0182981 ABC091 plate1 pass 395.0 0.0000492 9914.0 0.0177124 211.0 0.0000626 131.5 0.0007861 325.0 0.0000580 4608.0 0.0007312 1073.5 0.0002631 3021.0 0.0012034 ABC092 plate1 pass 904.5 0.0001198 1871.0 0.0017893 793.0 0.0002529 172.0 0.0009429 6106.0 0.0017829 1303.0 0.0002138 6595.5 0.0014839 2093.0 0.0007710 ABC093 plate1 pass 323.0 0.0000385 2007.5 0.0019218 1950.0 0.0005829 106.0 0.0006777 191.0 0.0000195 7377.5 0.0013278 1699.0 0.0003775 2411.0 0.0009095 ABC094 plate1 pass 706.0 0.0000950 2087.0 0.0020002 791.0 0.0002523 169.0 0.0009317 66.0 0.0000195 261.0 0.0000465 65.0 0.0000234 990.0 0.0003429 ABC095 plate1 pass 323.0 0.0000385 4950.0 0.0058606 264.0 0.0000829 107.0 0.0006821 645.0 0.0001415 8856.0 0.0017682 5330.0 0.0011204 7092.0 0.0061911 ABC096 plate1 pass 367.0 0.0000446 1067.0 0.0010494 259.0 0.0000812 101.0 0.0006552 7423.0 0.0022234 1841.5 0.0002925 335.0 0.0001074 1203.0 0.0004211 ABC097 plate2 pass 2712.0 0.0004324 1569.0 0.0015615 673.0 0.0001944 327.0 0.0013373 182.0 0.0000195 936.0 0.0001549 223.0 0.0000705 2208.0 0.0009189 ABC098 plate2 pass 134.0 0.0000215 378.0 0.0004445 117.0 0.0000238 197.0 0.0009164 58.0 0.0000195 122.0 0.0000222 93.0 0.0000274 290.0 0.0000850 ABC099 plate2 pass 182.0 0.0000229 209.0 0.0002515 208.0 0.0000444 374.0 0.0014804 221.5 0.0000195 293.0 0.0000512 868.0 0.0002201 376.0 0.0001154 ABC100 plate2 pass 152.0 0.0000220 229.5 0.0002764 101.0 0.0000227 89.0 0.0005012 48.0 0.0000195 109.0 0.0000217 110.0 0.0000345 103.0 0.0000239 ABC101 plate2 pass 1135.0 0.0001620 236.0 0.0002842 299.0 0.0000777 507.0 0.0018733 209.5 0.0000195 1665.5 0.0002609 266.0 0.0000819 650.0 0.0002165 ABC102 plate2 pass 174.0 0.0000226 395.0 0.0004627 175.0 0.0000348 78.0 0.0004511 70.0 0.0000195 294.5 0.0000515 92.0 0.0000273 293.5 0.0000862 ABC103 plate2 pass 421.0 0.0000514 2081.5 0.0020304 529.0 0.0001498 149.0 0.0007441 962.0 0.0002139 241.0 0.0000392 282.0 0.0000860 1632.5 0.0006324 ABC104 plate2 pass 24.0 0.0000195 49.0 0.0000338 22.0 0.0000195 13.0 0.0000597 15.5 0.0000195 28.0 0.0000195 17.0 0.0000195 14.0 0.0000195 ABC105 plate2 pass 24.0 0.0000195 45.0 0.0000280 22.0 0.0000195 13.0 0.0000597 16.0 0.0000195 29.0 0.0000195 17.0 0.0000195 13.0 0.0000195 ABC106 plate2 pass 21464.0 0.0057123 11789.0 0.0193731 2508.5 0.0007458 8001.0 0.0195240 2342.0 0.0006263 1992.0 0.0003077 2927.0 0.0006480 9995.5 0.0126203 ABC107 plate2 pass 795.0 0.0001092 6135.5 0.0073751 407.0 0.0001116 273.0 0.0011681 535.5 0.0000973 8821.0 0.0015565 998.0 0.0002476 7092.0 0.0052612 ABC108 plate2 pass 1574.0 0.0002334 348.5 0.0004125 892.0 0.0002615 288.0 0.0012157 306.0 0.0000428 315.0 0.0000562 366.0 0.0001070 548.0 0.0001781 ABC109 plate2 pass 146.0 0.0000218 408.5 0.0004771 1130.5 0.0003337 83.0 0.0004742 100.0 0.0000195 652.0 0.0001116 130.0 0.0000403 443.0 0.0001395 ABC110 plate2 pass 1330.5 0.0001933 3036.0 0.0029675 965.0 0.0002837 174.0 0.0008356 79.0 0.0000195 179.0 0.0000279 92.0 0.0000273 1092.0 0.0003929 ABC111 plate2 pass 358.0 0.0000396 4074.5 0.0041467 335.5 0.0000892 140.0 0.0007101 390.0 0.0000616 8814.0 0.0015547 5627.0 0.0012826 7093.0 0.0052626 ABC112 plate2 pass 481.0 0.0000611 1870.0 0.0018351 421.0 0.0001160 182.5 0.0008658 1777.5 0.0004552 2465.0 0.0003758 411.0 0.0001179 1832.0 0.0007279 ABC113 plate2 pass 551.0 0.0000731 1282.0 0.0013030 331.5 0.0000879 165.0 0.0008031 77.0 0.0000195 579.0 0.0001000 229.0 0.0000721 244.0 0.0000691 ABC114 plate2 pass 943.0 0.0001319 584.5 0.0006563 235.0 0.0000564 135.0 0.0006908 3017.0 0.0008320 4928.0 0.0007577 7730.0 0.0018913 5339.0 0.0032075 ABC115 plate2 pass 532.5 0.0000704 1533.0 0.0015290 315.0 0.0000827 210.0 0.0009610 2575.5 0.0006974 2779.0 0.0004215 7300.0 0.0017563 1029.0 0.0003668 ABC116 plate2 pass 768.0 0.0001051 12605.0 0.0200000 409.0 0.0001123 7632.0 0.0193657 9860.0 0.0029626 5612.0 0.0008768 6383.0 0.0014873 7201.0 0.0054162 ABC117 plate2 pass 130.0 0.0000214 239.5 0.0002884 72.0 0.0000195 64.0 0.0003835 37.0 0.0000195 204.0 0.0000319 49.0 0.0000220 156.0 0.0000368 ABC118 plate2 pass 40.0 0.0000195 60.0 0.0000508 37.0 0.0000195 25.0 0.0001517 26.5 0.0000195 40.0 0.0000195 27.0 0.0000195 23.5 0.0000195 ABC119 plate2 pass 167.0 0.0000224 605.0 0.0006764 253.0 0.0000617 329.0 0.0013435 3973.0 0.0011236 803.0 0.0001348 1670.0 0.0003865 2812.0 0.0012581 ABC120 plate2 pass 1879.0 0.0002849 4562.0 0.0047821 415.0 0.0001141 18287.0 0.0200000 11482.5 0.0035037 4830.5 0.0007413 4217.0 0.0009350 6291.0 0.0042299 ABC121 plate2 pass 256.0 0.0000272 1507.0 0.0015056 117.0 0.0000238 129.0 0.0006675 168.0 0.0000195 908.0 0.0001507 87.0 0.0000268 315.0 0.0000938 ABC122 plate2 pass 24.0 0.0000195 49.0 0.0000338 22.0 0.0000195 12.0 0.0000483 15.0 0.0000195 29.0 0.0000195 17.0 0.0000195 14.0 0.0000195 ABC123 plate2 pass 384.0 0.0000439 1708.0 0.0016873 326.0 0.0000862 1172.5 0.0038397 986.0 0.0002208 299.5 0.0000523 149.0 0.0000455 902.0 0.0003150 ABC124 plate2 pass 210.0 0.0000237 512.0 0.0005840 221.0 0.0000492 191.0 0.0008956 89.0 0.0000195 410.0 0.0000725 174.5 0.0000571 398.0 0.0001232 ABC125 plate2 pass 351.5 0.0000390 312.0 0.0003721 165.5 0.0000315 88.0 0.0004967 66.0 0.0000195 200.0 0.0000315 183.0 0.0000595 174.0 0.0000406 ABC126 plate2 pass 1278.0 0.0001848 5810.0 0.0067534 275.0 0.0000702 230.5 0.0010298 137.0 0.0000195 1762.0 0.0002748 1241.0 0.0002983 1903.7 0.0007632 ABC127 plate2 pass 318.0 0.0000360 397.0 0.0004649 130.0 0.0000246 130.5 0.0006734 78.0 0.0000195 1513.5 0.0002391 325.0 0.0000969 492.1 0.0001574 ABC128 plate2 pass 493.5 0.0000648 2980.5 0.0029098 401.0 0.0001097 5932.5 0.0178504 3536.0 0.0009903 574.0 0.0000993 5389.0 0.0012210 4699.5 0.0026207 ABC129 plate2 pass 9686.0 0.0019673 873.0 0.0009308 333.0 0.0000884 166.0 0.0008068 791.0 0.0001657 417.5 0.0000737 262.0 0.0000808 653.0 0.0002176 ABC130 plate2 pass 777.5 0.0001066 581.0 0.0006528 241.5 0.0000583 173.0 0.0008320 1479.5 0.0003657 1504.0 0.0002378 260.0 0.0000803 439.0 0.0001380 ABC131 plate2 pass 455.0 0.0000576 416.5 0.0004855 126.0 0.0000243 67.0 0.0003984 48.0 0.0000195 183.0 0.0000283 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pass 596.0 0.0000765 15139.0 0.0200000 371.5 0.0001185 229.0 0.0010876 535.5 0.0001075 7928.5 0.0015422 1414.5 0.0003336 8105.0 0.0082846 ABC226 plate3 pass 1028.0 0.0001324 2646.0 0.0027065 1486.0 0.0004513 292.0 0.0012714 9357.0 0.0028205 3736.0 0.0005969 14370.5 0.0056660 7769.5 0.0072079 ABC227 plate3 pass 512.0 0.0000659 4735.0 0.0056910 3408.0 0.0010010 127.5 0.0007666 191.0 0.0000195 11567.0 0.0030729 2923.5 0.0006547 901.0 0.0003305 ABC228 plate3 pass 883.0 0.0001133 1953.0 0.0019645 755.0 0.0002393 586.0 0.0020929 122.0 0.0000195 425.5 0.0000768 121.0 0.0000390 529.0 0.0001817 ABC229 plate3 pass 977.0 0.0001257 8719.0 0.0152000 509.0 0.0001632 187.0 0.0009599 1289.0 0.0003100 13891.0 0.0049382 9531.0 0.0026711 5039.5 0.0028202 ABC230 plate3 pass 791.0 0.0001014 1944.0 0.0019554 465.0 0.0001491 216.5 0.0010501 12307.0 0.0039802 4157.0 0.0006711 760.0 0.0001958 8907.0 0.0134354 ABC231 plate3 pass 397.0 0.0000456 987.0 0.0010277 924.0 0.0002897 133.0 0.0007853 79.0 0.0000195 2317.5 0.0003667 1080.5 0.0002639 253.0 0.0000745 ABC232 plate3 pass 1111.0 0.0001435 613.0 0.0006700 357.0 0.0001137 196.0 0.0009877 8496.0 0.0025127 5772.5 0.0009915 13202.0 0.0047167 7914.0 0.0076416 ABC233 plate3 pass 956.0 0.0001229 2369.0 0.0024001 644.0 0.0002054 389.0 0.0015451 8762.0 0.0026065 6437.0 0.0011435 21113.0 0.0114948 4649.0 0.0024719 ABC234 plate3 pass 1307.0 0.0001702 20590.0 0.0200000 1335.0 0.0004084 10780.0 0.0200000 12736.0 0.0041643 5493.0 0.0009314 7696.0 0.0019655 7375.5 0.0062016 ABC235 plate3 pass 433.0 0.0000528 382.0 0.0004380 173.0 0.0000449 105.0 0.0006872 59.0 0.0000195 655.0 0.0001144 367.0 0.0001072 599.0 0.0002094 ABC236 plate3 pass 161.0 0.0000229 279.0 0.0003280 2524.5 0.0007446 105.5 0.0006891 55.0 0.0000195 681.5 0.0001186 472.5 0.0001320 491.0 0.0001668 ABC237 plate3 pass 439.0 0.0000553 732.5 0.0007856 397.0 0.0001269 2108.0 0.0075580 9692.0 0.0029437 9513.0 0.0020840 7341.0 0.0018452 6115.0 0.0040204 ABC238 plate3 pass 1837.0 0.0002458 11406.0 0.0200000 467.5 0.0001499 20011.0 0.0200000 19606.0 0.0078560 5822.0 0.0010024 5127.5 0.0011877 5476.5 0.0032592 ABC239 plate3 pass 244.0 0.0000275 1665.5 0.0016775 166.0 0.0000430 124.0 0.0007545 154.0 0.0000195 718.0 0.0001244 115.0 0.0000377 224.0 0.0000618 ABC240 plate3 pass 482.0 0.0000603 3990.5 0.0044563 388.0 0.0001240 3736.0 0.0141769 7436.0 0.0021501 4111.5 0.0006629 2819.0 0.0006317 3361.0 0.0015428 ABC241 plate3 pass 656.0 0.0000841 3103.0 0.0032470 505.0 0.0001619 14386.0 0.0200000 2343.0 0.0006023 357.5 0.0000651 1215.0 0.0002920 2503.0 0.0010582 ABC242 plate3 pass 243.0 0.0000275 568.5 0.0006264 693.0 0.0002205 141.0 0.0008122 79.0 0.0000195 2080.0 0.0003305 1470.0 0.0003451 1295.0 0.0004941 ABC243 plate3 pass 1409.5 0.0001844 1123.0 0.0011563 379.0 0.0001210 153.0 0.0008518 167.5 0.0000195 1553.0 0.0002511 1738.0 0.0004009 1280.5 0.0004879 ABC244 plate3 pass 467.5 0.0000586 6741.0 0.0098601 238.0 0.0000730 174.0 0.0009191 181.0 0.0000195 1660.5 0.0002672 1087.0 0.0002653 1804.5 0.0007186 ABC245 plate3 pass 329.0 0.0000380 630.0 0.0006866 196.0 0.0000568 119.0 0.0007371 278.0 0.0000437 2052.5 0.0003263 2221.0 0.0005025 223.0 0.0000615 ABC246 plate3 pass 1122.5 0.0001451 17810.0 0.0200000 677.0 0.0002156 12254.0 0.0200000 16011.0 0.0057279 3551.0 0.0005652 10222.5 0.0029805 3142.0 0.0014107 ABC247 plate3 pass 10853.0 0.0024930 817.5 0.0008669 255.0 0.0000789 189.0 0.0009661 2247.5 0.0005755 590.0 0.0001040 258.0 0.0000801 1023.0 0.0003804 ABC248 plate3 pass 743.5 0.0000952 820.0 0.0008693 313.5 0.0000990 289.0 0.0012628 9351.5 0.0028185 3062.0 0.0004842 676.5 0.0001777 873.0 0.0003191 ABC249 plate3 pass 723.0 0.0000926 1255.0 0.0012815 299.0 0.0000941 253.0 0.0011585 95.0 0.0000195 396.0 0.0000717 129.5 0.0000409 203.0 0.0000545 ABC250 plate3 pass 168.0 0.0000231 684.0 0.0007389 131.0 0.0000291 198.0 0.0009938 135.5 0.0000195 292.0 0.0000522 254.5 0.0000792 223.4 0.0000616 ABC251 plate3 pass 180.0 0.0000234 276.0 0.0003247 205.0 0.0000598 4646.5 0.0168749 2550.0 0.0006605 724.0 0.0001254 2707.0 0.0006071 664.5 0.0002354 ABC252 plate3 pass 249.5 0.0000277 4104.0 0.0046296 250.0 0.0000772 96.0 0.0006541 68.0 0.0000195 960.0 0.0001619 672.0 0.0001767 563.0 0.0001952 ABC253 plate3 pass 479.0 0.0000600 190.0 0.0002270 105.0 0.0000245 131.0 0.0007785 66.0 0.0000195 1724.5 0.0002768 108.0 0.0000348 406.0 0.0001335 ABC254 plate3 pass 164.0 0.0000230 327.0 0.0003800 234.5 0.0000718 66.0 0.0005349 75.5 0.0000195 260.0 0.0000454 89.0 0.0000273 259.0 0.0000768 ABC255 plate3 pass 281.5 0.0000328 1794.0 0.0018046 413.0 0.0001322 75.0 0.0005723 469.5 0.0000906 345.0 0.0000629 744.5 0.0001925 530.5 0.0001823 ABC256 plate3 pass 269.0 0.0000320 1067.0 0.0011033 141.0 0.0000343 7595.0 0.0198877 14464.0 0.0049520 804.5 0.0001379 467.0 0.0001307 7592.0 0.0067261 ABC257 plate3 pass 3247.0 0.0004729 382.0 0.0004380 470.0 0.0001507 98.0 0.0006615 2319.5 0.0005957 1521.0 0.0002464 1258.0 0.0003010 2304.5 0.0009573 ABC258 plate3 pass 20593.0 0.0088057 14902.5 0.0200000 2021.0 0.0006021 10184.0 0.0200000 5805.5 0.0016233 4307.5 0.0006985 3073.0 0.0006880 14083.5 0.0184996 ABC259 plate3 pass 395.0 0.0000454 9914.0 0.0183875 211.0 0.0000618 131.5 0.0007802 325.0 0.0000548 4608.0 0.0007545 1073.5 0.0002624 2791.0 0.0012115 ABC260 plate3 pass 904.5 0.0001161 1871.0 0.0018816 793.0 0.0002507 172.0 0.0009128 6106.0 0.0017178 1303.0 0.0002137 6595.5 0.0016070 744.0 0.0002671 ABC261 plate3 pass 323.0 0.0000375 2007.5 0.0020201 1950.0 0.0005821 106.0 0.0006909 191.0 0.0000195 7377.5 0.0013845 1699.0 0.0003928 2411.0 0.0010110 ABC262 plate3 pass 706.0 0.0000904 2087.0 0.0021019 791.0 0.0002501 169.0 0.0009033 66.0 0.0000195 261.0 0.0000456 65.0 0.0000234 412.0 0.0001359 ABC263 plate3 pass 323.0 0.0000375 4950.0 0.0060963 264.0 0.0000820 107.0 0.0006945 645.0 0.0001361 8856.0 0.0018414 5330.0 0.0012421 2381.0 0.0009958 ABC264 plate3 pass 367.0 0.0000429 1067.0 0.0011033 259.0 0.0000803 101.0 0.0006726 7423.0 0.0021457 1841.5 0.0002944 335.0 0.0000994 1494.0 0.0005798

antigen Sample mfi dilution mfi_pred EBP S1 15710.5 1.0000000 14505.89812 EBP S2 13545.0 0.5000000 12716.96189 EBP S3 9767.0 0.2500000 10069.57397 EBP S4 5648.5 0.1250000 6886.17642 EBP S5 4104.5 0.0625000 3977.57496 EBP S6 2105.0 0.0312500 1995.67778 EBP S7 1107.0 0.0156250 933.47149 EBP S8 452.5 0.0078125 438.32642 EBP S9 209.0 0.0039063 216.96880 EBP S10 105.0 0.0019531 115.69107 LF005 S1 11990.0 1.0000000 11173.72239 LF005 S2 8285.0 0.5000000 8062.10447 LF005 S3 4950.0 0.2500000 5136.85494 LF005 S4 2519.0 0.1250000 2860.90160 LF005 S5 1431.0 0.0625000 1413.82538 LF005 S6 676.5 0.0312500 648.06533 LF005 S7 328.0 0.0156250 293.36895 LF005 S8 141.0 0.0078125 139.32366 LF005 S9 65.0 0.0039063 72.34048 LF005 S10 42.0 0.0019531 41.79359 LF010 S1 22583.0 1.0000000 20988.27919 LF010 S2 16947.5 0.5000000 16700.05282 LF010 S3 10865.0 0.2500000 11718.91923 LF010 S4 6060.0 0.1250000 7099.84801 LF010 S5 3711.5 0.0625000 3761.11766 LF010 S6 1889.0 0.0312500 1830.79833 LF010 S7 1106.0 0.0156250 873.50674 LF010 S8 465.0 0.0078125 430.79743 LF010 S9 206.5 0.0039063 226.48047 LF010 S10 113.5 0.0019531 128.40660 LF016 S1 21244.0 1.0000000 20180.85191 LF016 S2 16146.0 0.5000000 15638.73289 LF016 S3 10621.5 0.2500000 10886.52702 LF016 S4 5968.5 0.1250000 6677.59798 LF016 S5 3738.0 0.0625000 3601.24538 LF016 S6 1667.0 0.0312500 1749.81963 LF016 S7 890.0 0.0156250 805.19740 LF016 S8 405.5 0.0078125 372.87919 LF016 S9 181.5 0.0039063 183.12292 LF016 S10 87.0 0.0019531 98.48603 MSP8 S1 24306.0 1.0000000 21938.24358 MSP8 S2 17172.0 0.5000000 17176.29860 MSP8 S3 11358.0 0.2500000 11937.60536 MSP8 S4 6237.0 0.1250000 7276.82653 MSP8 S5 3883.5 0.0625000 3956.60371 MSP8 S6 2136.0 0.0312500 2002.59938 MSP8 S7 1204.0 0.0156250 992.19123 MSP8 S8 522.0 0.0078125 501.13680 MSP8 S9 249.0 0.0039063 264.50975 MSP8 S10 134.0 0.0019531 147.56635 RBP2b.P87 S1 7907.5 1.0000000 7684.71922 RBP2b.P87 S2 7186.0 0.5000000 6652.06667 RBP2b.P87 S3 4475.5 0.2500000 5197.61285 RBP2b.P87 S4 3508.0 0.1250000 3540.94573 RBP2b.P87 S5 2082.0 0.0625000 2089.09922 RBP2b.P87 S6 1102.0 0.0312500 1107.35922 RBP2b.P87 S7 657.0 0.0156250 561.79109 RBP2b.P87 S8 277.0 0.0078125 288.86874 RBP2b.P87 S9 138.0 0.0039063 155.88375 RBP2b.P87 S10 93.0 0.0019531 89.57501 PTEX150 S1 13207.0 1.0000000 11914.31017 PTEX150 S2 8550.0 0.5000000 8741.42263 PTEX150 S3 5329.0 0.2500000 5534.13998 PTEX150 S4 2671.0 0.1250000 3003.73525 PTEX150 S5 1548.0 0.0625000 1446.50976 PTEX150 S6 698.5 0.0312500 659.56391 PTEX150 S7 333.0 0.0156250 304.37262 PTEX150 S8 156.0 0.0078125 148.88984 PTEX150 S9 77.0 0.0039063 78.93516 PTEX150 S10 40.0 0.0019531 45.57518 PvCSS S1 12427.0 1.0000000 10908.34570 PvCSS S2 8034.0 0.5000000 8288.35265 PvCSS S3 4990.0 0.2500000 5333.02314 PvCSS S4 2446.5 0.1250000 2828.67060 PvCSS S5 1299.0 0.0625000 1274.17986 PvCSS S6 581.0 0.0312500 530.74131 PvCSS S7 264.0 0.0156250 224.94855 PvCSS S8 111.0 0.0078125 103.53211 PvCSS S9 46.5 0.0039063 53.15334 PvCSS S10 28.0 0.0019531 30.47789 antigen Sample mfi dilution mfi_pred EBP S1 17664.0 1.0000000 23002.27129 EBP S2 14051.0 0.5000000 14693.76571 EBP S3 8807.0 0.2500000 9027.18301 EBP S4 5744.0 0.1250000 5335.11108 EBP S5 3631.5 0.0625000 3036.86130 EBP S6 1751.5 0.0312500 1668.66109 EBP S7 1076.0 0.0156250 888.03624 EBP S8 433.0 0.0078125 459.79622 EBP S9 200.5 0.0039063 232.91585 EBP S10 116.0 0.0019531 116.19241 LF005 S1 13024.0 1.0000000 12314.66100 LF005 S2 9319.0 0.5000000 8906.21718 LF005 S3 4496.5 0.2500000 5507.65216 LF005 S4 2675.5 0.1250000 2893.92592 LF005 S5 1486.0 0.0625000 1347.47696 LF005 S6 664.0 0.0312500 602.24100 LF005 S7 347.0 0.0156250 280.03365 LF005 S8 137.0 0.0078125 142.64752 LF005 S9 76.0 0.0039063 81.10993 LF005 S10 44.0 0.0019531 51.31671 LF010 S1 24023.5 1.0000000 23319.61231 LF010 S2 18419.0 0.5000000 17399.67847 LF010 S3 9995.0 0.2500000 11528.10493 LF010 S4 6065.0 0.1250000 6779.52950 LF010 S5 4004.0 0.0625000 3632.24375 LF010 S6 1898.0 0.0312500 1853.50331 LF010 S7 1165.0 0.0156250 943.09885 LF010 S8 432.0 0.0078125 495.20043 LF010 S9 257.0 0.0039063 273.65449 LF010 S10 161.0 0.0019531 160.42518 LF016 S1 23687.0 1.0000000 22201.21470 LF016 S2 18294.0 0.5000000 16553.51159 LF016 S3 9094.5 0.2500000 11049.97381 LF016 S4 6029.0 0.1250000 6545.56744 LF016 S5 3673.0 0.0625000 3482.15636 LF016 S6 1746.0 0.0312500 1722.89720 LF016 S7 1052.0 0.0156250 832.67276 LF016 S8 346.0 0.0078125 412.35178 LF016 S9 211.0 0.0039063 216.64948 LF016 S10 121.5 0.0019531 122.99603 MSP8 S1 24512.0 1.0000000 24166.48961 MSP8 S2 20151.5 0.5000000 18907.32118 MSP8 S3 10652.5 0.2500000 12707.03017 MSP8 S4 6723.0 0.1250000 7385.28850 MSP8 S5 4293.5 0.0625000 3914.95062 MSP8 S6 2068.0 0.0312500 2011.61017 MSP8 S7 1346.0 0.0156250 1043.59085 MSP8 S8 522.0 0.0078125 557.66598 MSP8 S9 293.0 0.0039063 309.27964 MSP8 S10 167.0 0.0019531 178.29673 RBP2b.P87 S1 9648.0 1.0000000 9035.25758 RBP2b.P87 S2 6529.5 0.5000000 6909.87641 RBP2b.P87 S3 4671.0 0.2500000 4893.22895 RBP2b.P87 S4 3427.0 0.1250000 3188.66022 RBP2b.P87 S5 1910.0 0.0625000 1916.02277 RBP2b.P87 S6 1082.0 0.0312500 1074.89186 RBP2b.P87 S7 586.0 0.0156250 575.48015 RBP2b.P87 S8 283.0 0.0078125 302.23721 RBP2b.P87 S9 174.0 0.0039063 160.06631 RBP2b.P87 S10 86.0 0.0019531 87.46774 PTEX150 S1 14354.0 1.0000000 13601.80462 PTEX150 S2 9922.0 0.5000000 9482.86925 PTEX150 S3 4885.0 0.2500000 5776.41760 PTEX150 S4 2917.5 0.1250000 3079.27315 PTEX150 S5 1579.5 0.0625000 1483.48968 PTEX150 S6 725.5 0.0312500 682.74186 PTEX150 S7 370.0 0.0156250 318.36895 PTEX150 S8 138.0 0.0078125 157.26559 PTEX150 S9 76.0 0.0039063 84.29492 PTEX150 S10 52.5 0.0019531 49.36811 PvCSS S1 13146.5 1.0000000 12976.96364 PvCSS S2 9214.0 0.5000000 8521.30267 PvCSS S3 4300.0 0.2500000 4992.60176 PvCSS S4 2572.5 0.1250000 2617.17393 PvCSS S5 1393.0 0.0625000 1251.47652 PvCSS S6 537.0 0.0312500 564.62733 PvCSS S7 290.0 0.0156250 250.30884 PvCSS S8 98.5 0.0078125 113.27146 PvCSS S9 52.0 0.0039063 53.86393 PvCSS S10 29.0 0.0019531 27.39682 antigen Sample mfi dilution mfi_pred EBP S1 17357.0 1.0000000 17903.38228 EBP S2 11465.0 0.5000000 12954.34792 EBP S3 9607.0 0.2500000 8831.96646 EBP S4 5730.5 0.1250000 5639.50114 EBP S5 3695.0 0.0625000 3357.41442 EBP S6 1838.0 0.0312500 1859.79753 EBP S7 939.0 0.0156250 959.96721 EBP S8 446.5 0.0078125 464.42897 EBP S9 204.0 0.0039063 212.88241 EBP S10 98.0 0.0019531 93.91117 LF005 S1 12746.0 1.0000000 10888.10849 LF005 S2 7017.0 0.5000000 7919.29031 LF005 S3 4992.0 0.2500000 5013.60093 LF005 S4 2572.5 0.1250000 2745.97642 LF005 S5 1335.0 0.0625000 1339.36297 LF005 S6 644.5 0.0312500 616.80035 LF005 S7 297.0 0.0156250 286.10319 LF005 S8 169.5 0.0078125 140.37385 LF005 S9 67.5 0.0039063 74.76286 LF005 S10 39.0 0.0019531 43.53531 LF010 S1 22462.0 1.0000000 19976.47255 LF010 S2 15284.0 0.5000000 15762.92445 LF010 S3 10884.0 0.2500000 11096.85818 LF010 S4 6139.0 0.1250000 6835.54882 LF010 S5 3426.0 0.0625000 3705.00683 LF010 S6 1895.5 0.0312500 1835.27967 LF010 S7 953.0 0.0156250 881.34288 LF010 S8 604.0 0.0078125 434.60047 LF010 S9 214.0 0.0039063 228.88369 LF010 S10 100.0 0.0019531 131.08963 LF016 S1 21253.0 1.0000000 22243.83461 LF016 S2 14391.0 0.5000000 15237.69853 LF016 S3 10965.0 0.2500000 9724.26749 LF016 S4 5579.0 0.1250000 5776.93551 LF016 S5 3282.0 0.0625000 3207.29070 LF016 S6 1668.0 0.0312500 1678.74963 LF016 S7 773.0 0.0156250 839.18527 LF016 S8 511.5 0.0078125 406.95761 LF016 S9 185.0 0.0039063 194.64016 LF016 S10 84.0 0.0019531 93.26060 MSP8 S1 24716.5 1.0000000 21862.56575 MSP8 S2 16327.0 0.5000000 17309.53298 MSP8 S3 12073.0 0.2500000 12185.54243 MSP8 S4 6660.0 0.1250000 7505.75730 MSP8 S5 3843.5 0.0625000 4097.81480 MSP8 S6 2098.0 0.0312500 2070.17088 MSP8 S7 1130.0 0.0156250 1022.93664 MSP8 S8 724.0 0.0078125 518.16783 MSP8 S9 254.0 0.0039063 276.96855 MSP8 S10 126.0 0.0019531 158.18833 RBP2b.P87 S1 9629.0 1.0000000 8243.66588 RBP2b.P87 S2 6187.0 0.5000000 6772.38413 RBP2b.P87 S3 4284.0 0.2500000 5008.28387 RBP2b.P87 S4 3525.0 0.1250000 3276.95973 RBP2b.P87 S5 1943.0 0.0625000 1914.33459 RBP2b.P87 S6 969.0 0.0312500 1036.18532 RBP2b.P87 S7 573.0 0.0156250 545.78084 RBP2b.P87 S8 363.0 0.0078125 291.69153 RBP2b.P87 S9 152.5 0.0039063 162.38269 RBP2b.P87 S10 85.0 0.0019531 95.31063 PTEX150 S1 13067.0 1.0000000 11850.29398 PTEX150 S2 8013.0 0.5000000 8526.19240 PTEX150 S3 5278.0 0.2500000 5373.84943 PTEX150 S4 2869.0 0.1250000 2948.31175 PTEX150 S5 1449.5 0.0625000 1443.85077 PTEX150 S6 714.0 0.0312500 667.06947 PTEX150 S7 301.0 0.0156250 310.77740 PTEX150 S8 181.0 0.0078125 154.18782 PTEX150 S9 75.0 0.0039063 83.99597 PTEX150 S10 48.0 0.0019531 50.66502 PvCSS S1 13359.0 1.0000000 12505.65788 PvCSS S2 7865.5 0.5000000 8231.62584 PvCSS S3 4914.0 0.2500000 4874.02682 PvCSS S4 2554.0 0.1250000 2593.28693 PvCSS S5 1245.0 0.0625000 1255.27524 PvCSS S6 576.5 0.0312500 567.68297 PvCSS S7 236.0 0.0156250 248.91781 PvCSS S8 130.0 0.0078125 110.16134 PvCSS S9 47.0 0.0039063 50.97519 PvCSS S10 25.0 0.0019531 25.29125

QC Plots

Repeat the same steps as above to find the QC plots!

#### Standard Curve Plot
no_classification_final_analysis[[2]]

#### Bead Counts QC Plot
no_classification_final_analysis[[3]] # Plot

no_classification_final_analysis[[4]] # Samples to repeat 

# A tibble: 2 × 4
  Location SampleID Plate  QC   
  <chr>    <chr>    <chr>  <chr>
1 A1       Blank1   plate2 fail 
2 A2       Blank2   plate2 fail 

#### Blanks QC Plot
no_classification_final_analysis[[5]]

#### Model Output Plot
no_classification_final_analysis[[6]]

$plate1

$plate2

$plate3

Create a PDF Report

renderQCReport(
  your_raw_data, 
  your_plate_layout, 
  "magpix", 
  location = "ETH"
)

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  |...                                            |   7%                       
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  |......                                         |  13% [setup]               
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  |.........                                      |  20%                       
  |                                                     
  |.............                                  |  27% [standard curves plot]

  |                                                     
  |................                               |  33%                       
  |                                                     
  |...................                            |  40% [model results plot]  

  |                                                     
  |......................                         |  47%                       
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  |.........................                      |  53% [bead counts plot]    

  |                                                     
  |............................                   |  60%                       
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  |...............................                |  67% [check repeats table] 
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  |......................................         |  80% [blank samples plot]  

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  |.........................................      |  87%                       
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  |............................................   |  93% [plate layouts]       
  |                                                     
  |...............................................| 100%                       
                                                                                                                 

/Applications/RStudio.app/Contents/Resources/app/quarto/bin/tools/x86_64/pandoc +RTS -K512m -RTS template.knit.md --to latex --from markdown+autolink_bare_uris+tex_math_single_backslash --output /Users/Dionne/Documents/GitHub/pvsero/experiment1_20250730_ETH_v1.3.1_QCreport.tex --lua-filter /Library/Frameworks/R.framework/Versions/4.4-x86_64/Resources/library/rmarkdown/rmarkdown/lua/pagebreak.lua --lua-filter /Library/Frameworks/R.framework/Versions/4.4-x86_64/Resources/library/rmarkdown/rmarkdown/lua/latex-div.lua --embed-resources --standalone --highlight-style tango --pdf-engine pdflatex --variable graphics --include-in-header /var/folders/bh/0yzt0_x97vj_zktb_39c1xvh0000gn/T//RtmpbXTf0U/rmarkdown-str167875bdf55bc.html --variable 'geometry:margin=1in' --include-in-header /var/folders/bh/0yzt0_x97vj_zktb_39c1xvh0000gn/T//RtmpbXTf0U/rmarkdown-str167872375d8c3.html 

Data Analysis: Pk/Pv/Pf

5-Point Standard Curve

Step 1: Load your data!

Firstly, we will be using our example data that’s in-built in the package. Here replace the system.file() argument with the file path for your package.

your_raw_data_5std <- c(
  system.file("extdata", "example_MAGPIX_pk_5std_plate1.csv", package = "pvsero"),
  system.file("extdata", "example_MAGPIX_pk_5std_plate2.csv", package = "pvsero")
)
your_plate_layout_5std <- system.file("extdata", "example_platelayout_pk_5std.xlsx", package = "pvsero")

Step 2: Read your data and process MFI to RAU

Caitlin and Dionne have worked on a function to (a) process raw Serological data and (b) convert MFI to RAU. The runPlasmoSero5point() function will output three data frames: (i) Results from MAGPIX (MFI), (ii), Counts for each sample, (iii) Processed RAU values for each sample. These dataframes can be used for further analysis and can be transformed as you wish.

pk_analysis_1 <- runPlasmoSero5point(
  raw_data = your_raw_data_5std, 
  platform = "magpix", 
  plate_layout = your_plate_layout_5std, 
  date = "2025-07-30" # Optional: This will default to today's date
)

Standard Curve Plot: One Curve

As requested, this is a plot of ONE standard curve which you will specify. You can modify this plot as you see fit by piping other ggplot2() arguments.

plotOneStdCurve(pk_analysis_1, "plate1")

Standard Curve Plot: Compare Two Curves

This function allows you to plot all of your standard curves.

plotManyStdCurves(pk_analysis_1)

10-Point Standard Curve

These steps are very similar to the 5-point standard curve, except here we use the runPlasmoSero10point() function.

Step 1: Load your data!

your_raw_data_10std <- c(
  system.file("extdata", "example_MAGPIX_pk_10std_plate1.csv", package = "pvsero"),
  system.file("extdata", "example_MAGPIX_pk_10std_plate2.csv", package = "pvsero")
)
your_plate_layout_10std <- system.file("extdata", "example_platelayout_pk_10std.xlsx", package = "pvsero")

Step 2: Read your data and process MFI to RAU

pk_analysis_2 <- runPlasmoSero10point(
  raw_data = your_raw_data_10std, 
  platform = "magpix", 
  plate_layout = your_plate_layout_10std, 
  date = "2025-07-30" # Optional: This will default to today's date
)

Standard Curve Plot: One Curve

plotOneStdCurve(pk_analysis_2, "plate1")

Standard Curve Plot: Compare Two Curves

plotManyStdCurves(pk_analysis_2)

Visualisation of the {pvsero} R Package:

We have used the {targets} R package to generate a pipeline! This allows us to:

• Automatically detect the dependencies of each step
• One-command execution
• Automatic caching
• Automatic detection of changes in data and/or code

For more information on {targets} see this tutorial.

library(visNetwork)

tar_config_set(store = "../_targets", script = "../_targets.R")
tar_make()  # optional: run pipeline

here() starts at /Users/Dionne/Documents/GitHub/pvsero
✔ skipped pipeline [41ms, 15 skipped]
Warning message:
package ‘targets’ was built under R version 4.4.1 

network <- tar_network()

here() starts at /Users/Dionne/Documents/GitHub/pvsero
Warning message:
package ‘targets’ was built under R version 4.4.1 

visNetwork::visNetwork(nodes = network$nodes, edges = network$edges)